PAICS (NM_001079524) Human Untagged Clone

CAT#: SC323928

PAICS (untagged)-Human phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAICS
• Synonyms: ADE2; ADE2H1; AIRC; PAIS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001079524.1 GAAAGCTGTATTTGCTGCACGTGGAAATCTCCGTTATTTTCCAGCACCCAACAGTAGCGT
  AATGGGAGTAACGGACTTAACCTCATTTCTCTTTCAGAGCATTTAGCCTTCATATGCCCT
  TCCCTGCATGCTTCCCCCAGGCCGTCAAGACTTGAGTTCTGCCTCGCTTCCCGGCGCGGT
  CGCAGCCCTCAGCCCACTTAGGATAATGGCGACAGCTGAGGTACTGAACATTGGTAAAAA
  ATTATATGAGGGTAAAACAAAAGAAGTCTACGAATTGTTAGACAGTCCAGGAAAAGTCCT
  CCTGCAGTCCAAGGACCAGATTACAGCAGGAAATGCAGCTAGAAAAAACCACCTGGAAGG
  AAAAGCTGCAATCTCAAATAAAATCACCAGTTGTATTTTTCAGTTATTACAGGAAGCAGG
  TATTAAAACTGCCTTCACCAGAAAATGTGGGGAGACAGCTTTCATTGCACCGCAGTGTGA
  AATGATTCCAATTGAATGGGTTTGCAGAAGAATAGCAACTGGTTCTTTTCTCAAAAGAAA
  TCCTGGTGTCAAGGAAGGATATAAGTTTTACCCACCTAAAGTGGAGTTGTTTTTCAAGGA
  TGATGCCAATAATGACCCACAGTGGTCTGAGGAACAGCTGATTGCTGCAAAATTTTGCTT
  TGCTGGACTTCTTATAGGCCAGACTGAAGTGGATATCATGAGTCATGCTACACAGGCTAT
  ATTTGAAATACTGGAGAAATCCTGGTTGCCCCAGAATTGTACACTGGTTGATATGAAGAT
  TGAATTTGGTGTTGATGTAACCACCAAAGAAATTGTTCTTGCTGATGTTATTGACAATGA
  TTCCTGGAGACTCTGGCCATCAGGAGATCGAAGCCAACAGAAAGACAAACAGTCTTATCG
  GGACCTCAAAGAAGTAACTCCTGAAGGGCTCCAAATGGTAAAGAAAAACTTTGAGTGGGT
  TGCAGAGAGAGTAGAGTTGCTTTTGAAATCAGAAAGTCAGTGCAGGGTTGTAGTGTTGAT
  GGGCTCTACTTCTGATCTTGGTCACTGTGAAAAAATCAAGAAGGCCTGTGGAAATTTTGG
  CATTCCATGTGAACTTCGAGTAACATCTGCGCATAAAGGACCAGATGAAACTCTGAGGAT
  TAAAGCTGAGTATGAAGGGGATGGCATTCCTACTGTATTTGTGGCAGTGGCAGGCAGAAG
  TAATGGTTTGGGACCAGTGATGTCTGGGAACACTGCATATCCAGTTATCAGCTGTCCTCC
  CCTCACACCAGACTGGGGAGTTCAGGATGTGTGGTCTTCTCTTCGACTACCCAGTGGTCT
  TGGCTGTTCAACCGTACTTTCTCCAGAAGGATCAGCTCAATTTGCTGCTCAGATATTTGG
  GTTAAGCAACCATTTGGTATGGAGCAAACTGCGAGCAAGCATTTTGAACACATGGATTTC
  CTTGAAGCAGGCTGACAAGAAAATCAGAGAATGTAATTTATAAGAAAGAATGCCATTGAA
  TTTTTTAGGGGAAAAACTACAAATTTCTAATTTAGCTGAAGGAAAATCAAGCAAGATGAA
  AAGGTAATTTTAAATTAGAGAACACAAATAAAATGTATTAGTGAATAAATGCTTCTCTAG
  ATCCATATTAATAAACATGAGCATCTAACCCCTCCTTTCTTAGGCTAGACACCAAGATAT
  TTCAGCCAGCCTTTATCATTCCTCTTACTTTATCCTTTTTCCTTAAGTATTGGTGGTCAC
  TACTATTGAGTTTCTTCCTTAACACTGATTAAATGATCTTAACTCCCTCAGCTAAAACTG
  GCATTACTGACTCCCAGCTATATTTCTCCAGACTTGCATTTTTTTTTTTTTTTTTGAGAC
  AGGGTCTCACTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCAGTTCACTGCTGCTTT
  CCCTCCTGGGCTCAAGCAGTTCTCCCACCTCAGCCTCTCGACTAACAGGGACTATAATCT
  TGCAGCACCATGCCGAGCTAATTTTATTTTTTGTAGAGATGAGCTCTCACTATGTCACCC
  AGGTTCGTCTCAAACTCCTGAACCCTAGTAATTCTCCTATCTCAGCCTCCCAAAGTGCTA
  GGGTTACAGACATGAGCCACTGTGCCTGTCTAGACTTGTACTTTCAACTGTCCATTTCTC
  CCTGTCTGTCCCATGGGCACTCATGAAAAAACAGAATGCTCCCAACTTTATTCATCTTCC
  AAGCCTGTAGCTCTTGGTATACTCACTGTTGCAAGTCAGAAGCTTGATTTCATCATTGAT
  GTTTTTCTCACGTTTCACATCTCACTCATCACCAAGTCATGTTGGTGTTAATTTCTGATT
  AACCCTTGAATTTACCGTCTTCTCATCCTCTGTACAAAAGCCTCAAGTGAGGGTCAAATT
  CAACATTATCCTGATCTAGACAGCCCCCATTCTCAATCCACCCTTTTCCAAGTTGATTGC
  CCAAGGACTTCTAACAATAAACTCTCTTTTGCACCACAGACTTCTTTGAAAATATACATG
  CTGTTGACCCTCTCTGTAGAAAACCGCACACATAAAACTTACCAACAGATTTCATTGGTT
  CTTGGGTTCTCCCGAAGCCTATCCATGGTTTATAGATTAAGAATTGATGAGGTAGCTGGG
  CACAGTGGCTCACACCTACGATCACAGCACTTCGGGAGGCTGAAGCAAGCAGATCACTTG
  AGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAATA
  CAAAAAGTAGCCAGCCGTGATGACAGGCACCTGTAATCCCAGCTACTCGGGAGGCTGAGG
  CATGAGAATTGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCTGGATCATGCCACTGC
  ACTCCAACCTGGGCAGCAGAGCAAGACTCTGTCTCAAAAGGGGAAAAAAAAAATTGCTGA
  TGTGACCCATGAAGGGAACTCATTTTCCTCGTAATTTTGGACTGCCACACATTGGTACCT
  TTAGTTCTCTGAAGGCCCACGTTTTTATCATTAAGACCTATTTGTTAGCTAGTAGAGCTT
  TATGTTCGCTGTCCATGAAACCTTCTGTAACCACAGTGACTACAAGTAGTTCTTTCTCTA
  TTGAATTATTAGGTCCAGAATAGAAGATGTCATTGTACACTTTATTTCCCTCACACTGTG
  TTATGCTCTGATGTGCTATGCTTAGCTATCTGTCAGAGATTAGTAAATTATAAAACTCAT
  GTGTACTACTTAAGTTTATATCTTATGCTAGTTTATAAGAACAATTAAAAGGACTTAGAA
  GATTAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001079524.1 unedited
  TGCCATTTGTATACGACTCCTATAGGGCGGCCGGCGATCGCGGCGCGCCGGTACCGGTCC
  GGAATTCCCGGGATATCGTCGACCCACGCGTCCGGAAAGCTGTATTTGCTGCACGTGGAA
  ATCTCCGTTATTTTCCAGCACCCAACAGTAGCGTAATGGGAGTAACGGACTTAACCTCAT
  TTCTCTTTCAGAGCATTTAGCCTTCATATGCCCTTCCCTGCATGCTTCCCCCAGGCCGTC
  AAGACTTGAGTTCTGCCTCGCTTCCCGGCGCGGTCGCAGCCCTCAGCCCACTTAGGATAA
  TGGCGACAGCTGAGGTACTGAACATTGGTAAAAAATTATATGAGGGTAAAACAAAAGAAG
  TCTACGAATTGTTAGACAGTCCAGGAAAAGTCCTCCTGCAGTCCAAGGACCAGATTACAG
  CAGGAAATGCAGCTAGAAAAAACCACCTGGAAGGAAAAGCTGCAATCTCAAATAAAATCA
  CCAGTTGTATTTTTCAGTTATTACAGGAAGCAGGTATTAAAACTGCCTTCACCAGAAAAT
  GTGGGGAGACAGCTTTCATTGCACCGCAGTGTGAAATGATTCCAATTGAATGGGTTTGCA
  GAAGAATAGCAACTGGTTCTTTTCTCAAAAGAAATCCTGGTGTCAAGGAAGGATATAAGT
  TTTACCCACCTAAAGTGGAGTTGTTTTTCAAGGATGATGCCAATAATGACCCACAGTGGT
  CTGAGGAACAGCTGATTGCTGCAAAATTTTGCTTTGCTGGACTTCTTATAGGCCAGACTG
  AAGTGGATATCATGAGTCATGCTACACAGGCTATTATTTGAAAATACTGGAGAAATCCTG
  TTGCCCCAGAATTGTACAACTGGTTGATATGAGATTGATTTGGTGTGATGTAACCACCAA
  GAAATTGTCTTGCTGATGTTTATTGACAATGATTCCTGAGAACTCTGCATCAGGAATCGG
  AGCAATGAAGACAAAAGTTCTTATCCGGACTCTACAGGAAAGTAAC
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001079524
• ORF Size: 1278 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001079524.1, NP_001072992.1
• RefSeq Size: 3329
• RefSeq ORF: 1278
• Locus ID: 10606
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• Gene Summary: This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the CDS, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Variants 2 and 3 encode the same isoform.