Neuromedin B (NMB) (NM_021077) Human Untagged Clone

CAT#: SC324393

NMB (untagged)-Human neuromedin B (NMB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NMB
• Synonyms: MGC2277; MGC3936; MGC17211
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021077.3 GAAGAGAGGCTCAGAGGGCTGAAGTGCCTATTTGGCCGAAAGCCGTGGCAGAGTGGCAAG
  GCAGGGCCAGGGGAAGCGGCTCCGCCGCCGGGGCCGGGCCCCTGTTTGGCCCGGTGCCCG
  GTCCTTAGCCTGAAGGTGGCGGGCTTCCGCCAGAAGCCCCTGGCGGAAGCGGTGCCCGCG
  TGCGGGCCAGAGTGTGGGTGTGCAGGTCTCTGGGCGGCCCAAAGGGGGTGCCCCTGCCTG
  GTAACCTAGCGGGAGGGTGGGGACGGCGGGGAGGGCGGCGGGCGCGGGGCACGGCTCCGC
  TGCTCAGGGCAGGCTCCGCCCCCAGGGGCGCGGATTTAAAAGGATCGAAGGCAGCCCCGG
  AGCCCAGCGGCCGGGAGGCGCGCCCGAACGAAGCCGCGGCCCGGGCACAGCCATGGCCCG
  GCGGGCGGGGGGCGCTCGGATGTTCGGCAGCCTCCTGCTCTTCGCCCTGCTCGCTGCCGG
  CGTCGCCCCGCTCAGCTGGGATCTCCCGGAGCCCCGCAGCCGAGCCAGCAAGATCCGAGT
  GCACTCGCGAGGCAACCTCTGGGCCACCGGTCACTTCATGGGCAAGAAGAGTCTGGAGCC
  TTCCAGCCCATCCCCATTGGGGACAGCTACCCACACCTCCCTGAGGGACCAGCGACTGCA
  GCTGAGTCATGATCTGCTCGGAATCCTCCTGCTAAAGAAGGCTCTGGGCGTGAGCCTCAG
  CCGCCCCGCACCCCAAATCCAGGAGGCTGCTGGTACAAATACTGCAGAAATGACACCAAT
  AATGGGGCAGACACAACAGCGTGGCTTAGATTGTGCCCACCCAGGGAAGGTGCTGAATGG
  GACCCTGTTGATGGCCCCATCTGGATGTAAATCCTGAGCTCAAATCTCTGTTACTCCATT
  ACTGTGATTTCTGGCTGGGTCACCAGAAATATCGCTGATGCAGACACAGATTATGTTCCT
  GCTGTATTTCCTGCTTCCCTGTTGAATTGGTGAATAAAACCTTGCTCTTTACATACAAAA
  AAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_021077
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021077.3, NP_066563.2
• RefSeq Size: 1046 bp
• RefSeq ORF: 366 bp
• Locus ID: 4828
• Cytogenetics: 15q25.2
• Domains: Bombesin
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: 'This gene encodes a member of the bombesin-like family of neuropeptides, which negatively regulate eating behavior. The encoded protein may regulate colonic smooth muscle contraction through binding to its cognate receptor, the neuromedin B receptor (NMBR). Polymorphisms of this gene may be associated with hunger, weight gain and obesity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]'
  Transcript Variant: This variant (1) represents the longer transcript but encodes the shorter isoform (1).