Glutathione Peroxidase 2 (GPX2) (NM_002083) Human Untagged Clone

CAT#: SC324551

GPX2 (untagged)-Human glutathione peroxidase 2 (gastrointestinal) (GPX2) (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Human Untagged Clone
• Symbol: GPX2
• Synonyms: GI-GPx; GPRP; GPRP-2; GPx-2; GPx-GI; GSHPx-2; GSHPX-GI
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002083.2 GGGGGCTCACTCTGCGCTTCACCATGGCTTTCATTGCCAAGTCCTTCTATGACCTCAGTG
  CCATCAGCCTGGATGGGGAGAAGGTAGATTTCAATACGTTCCGGGGCAGGGCCGTGCTGA
  TTGAGAATGTGGCTTCGCTCTGAGGCACAACCACCCGGGACTTCACCCAGCTCAACGAGC
  TGCAATGCCGCTTTCCCAGGCGCCTGGTGGTCCTTGGCTTCCCTTGCAACCAATTTGGAC
  ATCAGGAGAACTGTCAGAATGAGGAGATCCTGAACAGTCTCAAGTATGTCCGTCCTGGGG
  GTGGATACCAGCCCACCTTCACCCTTGTCCAAAAATGTGAGGTGAATGGGCAGAACGAGC
  ATCCTGTCTTCGCCTACCTGAAGGACAAGCTCCCCTACCCTTATGATGACCCATTTTCCC
  TCATGACCGATCCCAAGCTCATCATTTGGAGCCCTGTGCGCCGCTCAGATGTGGCCTGGA
  ACTTTGAGAAGTTCCTCATAGGGCCGGAGGGAGAGCCCTTCCGACGCTACAGCCGCACCT
  TCCCAACCATCAACATTGAGCCTGACATCAAGCGCCTCCTTAAAGTTGCCATATAGATGT
  GAACTGCTCAACACACAGATCTCCTACTCCATCCAGTCCTGAGGAGCCTTAGGATGCAGC
  ATGCCTTCAGGAGACACTGCTGGACCTCAGCATTCCCTTGATATCAGTCCCCTTCACTGC
  AGAGCCTTGCCTTTCCCCTCTGCCTGTTTCCTTTTCCTCTCCCAACCCTCTGGTTGGTGA
  TTCAACTTGGGCTCCAAGACTTGGGTAAGCTCTGGGCCTTCACAGAATGATGGCACCTTC
  CTAAACCCTCATGGGTGGTGTCTGAGAGGCGTGAAGGGCCTGGAGCCACTCTGCTAGAAG
  AGACCAATAAAGGGCAGGTGTGGAAACGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002083
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002083.2, NP_002074.2
• RefSeq Size: 1024 bp
• Locus ID: 2877
• Cytogenetics: 14q23.3
• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Glutathione metabolism
• Gene Summary: 'The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is predominantly expressed in the gastrointestinal tract (also in liver in human), is localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. Overexpression of this gene is associated with increased differentiation and proliferation in colorectal cancer. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]'
  Transcript Variant: This variant (1) represents the protein-coding transcript.