Slap (SLA) (NM_001045556) Human Untagged Clone

CAT#: SC324569

SLA (untagged)-Human Src-like-adaptor (SLA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLA
• Synonyms: SLA1; SLAP
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001045556.1 GCCAGAAAAATGATGCACGATGCTCTCTAAACTGGGTCATTCTCCACTTGGAGGGCTCAG
  GGCACGGTTGACTTTCCCCGTCTGTCTCCTATACCACAGGCTCTGGGCATCACCAGCGGC
  CCCAGGGAAAAAGAAAGAAATGGGAAACAGCATGAAATCCACCCCTGCGCCTGCCGAGAG
  GCCCCTGCCCAACCCGGAGGGACTGGATAGCGACTTCCTTGCCGTGCTAAGTGACTACCC
  GTCTCCTGACATCAGCCCCCCGATATTCCGCCGAGGGGAGAAACTGCGTGTGATTTCTGA
  TGAAGGGGGCTGGTGGAAAGCTATTTCTCTTAGCACTGGTCGAGAGAGTTACATCCCTGG
  AATATGTGTGGCCAGAGTTTACCATGGCTGGCTGTTTGAGGGCCTGGGCAGAGACAAGGC
  CGAGGAGCTGCTGCAGCTGCCAGACACAAAGGTCGGCTCCTTTATGATCAGAGAGAGTGA
  GACCAAGAAAGGGTTTTACTCACTGTCGGTGAGACACAGGCAGGTAAAGCATTACCGCAT
  TTTCCGTCTGCCCAACAACTGGTACTACATTTCCCCGAGGCTCACCTTCCAGTGCCTGGA
  GGACCTGGTGAACCACTATTCTGAGGTGGCTGATGGCCTGTGCTGTGTGCTCACCACGCC
  CTGCCTGACACAAAGCACGGCTGCCCCAGCAGTGAGGGCCTCCAGCTCACCTGTCACCTT
  GCGTCAGAAGACTGTGGACTGGAGGAGAGTGTCCAGACTGCAGGAGGACCCCGAGGGAAC
  AGAGAACCCGCTTGGGGTAGACGAGTCCCTTTTCAGCTATGGCCTTCGAGAGAGCATTGC
  CTCTTACCTGTCCCTGACCAGTGAGGACAACACCTCCTTTGATCGAAAGAAGAAAAGCAT
  CTCCCTGATGTATGGTGGCAGCAAGAGAAAGAGCTCATTCTTCTCATCACCACCTTACTT
  TGAGGACTAGCCAAGAACAGACACAATGGTTCATGCCCAAAAGGAACAGAAGTTCCAACT
  ATTGCCTGGGATCTTGCGAAAAGCGAGGTTCCCTGATCCCTGGGAGCCTCACGTATTTTA
  GAAGCCAAGAGAAGCCACATGGAGACTCAAATTCGCATCTTCTCTATCCACATCATGACC
  AAAGGAACCCCTCCCTGGTGTCTGATCAGGGCTGTGGCATCACGAAACATTGGATCATGA
  CATGTCGGGCGATGCTTGGAAGAGCCCAGCATGTATGTATGCACACATTGTGTGTGTGGG
  AAGGACAAAGCCACTCTCACAAGAAAGGGCACCAGGACTGCTCTCCAAGGAACTGGACCT
  GTCCAGACAGTTACACTCCAAGGTCATTGGAGAGAACTTCTGTATGGGCAAGCCTGAGAG
  GGAGAGGAAACAAAAGCTGTGTCCTGGCAGAAGGTCTGGGTTTGCAGATGGGTGCCCTGA
  ATGGAACTACTTTAACTAATCCATAGGGACTTCTGGTATGCTTTCCTCTCTTTTTAAAGG
  AACTTCGTGACACTAAACATTAGCCCAAAGGACTTCTTAGCCTTCAATTGGGAGATACCT
  TTGGTCTGCTCCTGCACCAAAGCCATATGGGTGGAAGTCAGTTGGCCTCCCTGGTTCTGC
  AGAGGGCCAGAAGAATGAGAGAGAGGAAGACTGCTGGCAGGGAAATCGAGGAGGCGAGAC
  TAGAACTGCACCAGCTTCCCTGATGTCTGCAGCCATGGCTTTGCAGCGCAGACAGAGCTT
  CTCTGGGATGCTGGGATTCTTGCCTGTATGAATGCATCAAGTATTCATTTATTGCCCGAA
  TAGGCATTGCATTAAGTCCTCTGTAAGGTGTCAGGCAAGCAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001045556
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001045556.1, NP_001039021.1
• RefSeq Size: 3446 bp
• RefSeq ORF: 831 bp
• Locus ID: 6503
• Cytogenetics: 8q24.22
• Protein Families: Druggable Genome
• Gene Summary: ''
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform (a). CCDS Note: This CCDS represents a 276 aa protein that is encoded by NM_001045556.2, which is based on BX648382.1 and several locally supporting ESTs. However, it should be noted that NM_001045556.2 contains an upstream translation start codon at nts 504-506, as used in the NM_001045557.2 variant which encodes a 293 aa protein. The use of this upstream start codon in NM_001045556.2 would result in only a 15 aa ORF with a stop codon in the second exon. The short length of this upstream ORF may allow escape from nonsense-mediated mRNA decay (NMD) and translation reinitiation at the downstream start codon (nts 833-835) to encode the represented 276 aa protein, or alternatively, the weak Kozak signal at the upstream start codon may enable leaky scanning by the translation machinery and subsequent use of the downstream start codon. The 276 aa protein is consistent with reports in the literature, including PMIDs:7543898, 8825655 and 9660183. Variants encoding both the longer and shorter isoforms are also present in mouse (NM_001029841.3 and NM_009192.2, respectively).