Properdin (CFP) (NM_001145252) Human Untagged Clone

CAT#: SC325124

CFP (untagged)-Human complement factor properdin (CFP), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CFP
• Synonyms: BFD; PFC; PFD; PROPERDIN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001145252, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATCACAGAGGGAGCGCAGGCCCCTCGATTGTTGCTGCCGCCGCTGCTCCTGCTGCTCACCCTGCCAG
  CCACAGGCTCAGACCCCGTGCTCTGCTTCACCCAGTATGAAGAATCCTCCGGCAAGTGCAAGGGCCTCCT
  GGGGGGTGGTGTCAGCGTGGAAGACTGCTGTCTCAACACTGCCTTTGCCTACCAGAAACGTAGTGGTGGG
  CTCTGTCAGCCTTGCAGGTCCCCACGATGGTCCCTGTGGTCCACATGGGCCCCCTGTTCGGTGACGTGCT
  CTGAGGGCTCCCAGCTGCGGTACCGGCGCTGTGTGGGCTGGAATGGGCAGTGCTCTGGAAAGGTGGCACC
  TGGGACCCTGGAGTGGCAGCTCCAGGCCTGTGAGGACCAGCAGTGCTGTCCTGAGATGGGCGGCTGGTCT
  GGCTGGGGGCCCTGGGAGCCTTGCTCTGTCACCTGCTCCAAAGGGACCCGGACCCGCAGGCGAGCCTGTA
  ATCACCCTGCTCCCAAGTGTGGGGGCCACTGCCCAGGACAGGCACAGGAATCAGAGGCCTGTGACACCCA
  GCAGGTCTGCCCCACACACGGGGCCTGGGCCACCTGGGGCCCCTGGACCCCCTGCTCAGCCTCCTGCCAC
  GGTGGACCCCACGAACCTAAGGAGACACGAAGCCGCAAGTGTTCTGCACCTGAGCCCTCCCAGAAACCTC
  CTGGGAAGCCCTGCCCGGGGCTAGCCTACGAGCAGCGGAGGTGCACCGGCCTGCCACCCTGCCCAGTGGC
  TGGGGGCTGGGGGCCTTGGGGCCCTGTGAGCCCCTGCCCTGTGACCTGTGGCCTGGGCCAGACCATGGAA
  CAACGGACGTGCAATCACCCTGTGCCCCAGCATGGGGGCCCCTTCTGTGCTGGCGATGCCACCCGGACCC
  ACATCTGCAACACAGCTGTGCCCTGCCCTGTGGATGGGGAGTGGGACTCGTGGGGGGAGTGGAGCCCCTG
  TATCCGACGGAACATGAAGTCCATCAGCTGTCAAGAAATCCCGGGCCAGCAGTCACGCGGGAGGACCTGC
  AGGGGCCGCAAGTTTGACGGACATCGATGTGCCGGGCAACAGCAGGATATCCGGCACTGCTACAGCATCC
  AGCACTGCCCCTTGAAAGGATCATGGTCAGAGTGGAGTACCTGGGGGCTGTGCATGCCCCCCTGTGGACC
  TAATCCTACCCGTGCCCGCCAGCGCCTCTGCACACCCTTGCTCCCCAAGTACCCGCCCACCGTTTCCATG
  GTCGAAGGTCAGGGCGAGAAGAACGTGACCTTCTGGGGGAGACCGCTGCCACGGTGTGAGGAGCTACAAG
  GGCAGAAGCTGGTGGTGGAGGAGAAACGACCATGTCTACACGTGCCTGCTTGCAAAGACCCTGAGGAAGA
  GGAACTCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145252
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145252.1, NP_001138724.1
• RefSeq Size: 1669 bp
• RefSeq ORF: 1410 bp
• Locus ID: 5199
• Cytogenetics: Xp11.23
• Protein Families: Secreted Protein
• Gene Summary: 'This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Feb 2009]'
  Transcript Variant: This variant (2) differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Both variants 1 and 2 encode the same protein.