TBXAS1 (NM_001130966) Human Untagged Clone

CAT#: SC325170

TBXAS1 (untagged)-Human thromboxane A synthase 1 (platelet) (TBXAS1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBXAS1
• Synonyms: BDPLT14; CYP5; CYP5A1; GHOSAL; THAS; TS; TXAS; TXS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001130966, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATGGAAGCCTTGGGGTTTCTAAAATTGGAAGTGAATGGCCCCATGGTGACGGTGGCCCTGTCAGTGG
  CTCTCTTGGCCCTCCTGAAATGGTACTCCACATCAGCATTCTCAAGACTGGAGAAGTTAGGCCTCAGACA
  TCCCAAGCCTTCTCCTTTCATTGGAAACTTGACATTTTTCCGCCAGGGTTTTTGGGAAAGCCAAATGGAG
  CTCAGAAAGCTGTATGGACCTCTGTGTGGGTACTATCTTGGTCGTCGGATGTTTATTGTTATTTCTGAGC
  CAGACATGATCAAGCAGGTGTTGGTTGAGAACTTCAGTAACTTTACCAACAGAATGGCGTCGGGTTTGGA
  GTTCAAGTCGGTAGCCGACAGCGTTCTGTTTTTACGTGACAAAAGATGGGAAGAGGTCAGAGGTGCCCTG
  ATGTCTGCTTTCAGTCCTGAAAAGCTGAACGAGATGGTTCCCCTCATCAGCCAAGCCTGCGACCTTCTCC
  TGGCTCATTTAAAACGCTATGCGGAATCTGGGGACGCATTTGACATCCAGAGGTGCTACTGCAATTACAC
  CACAGATGTGGTTGCCAGCGTCGCCTTTGGCACCCCGGTGGACTCCTGGCAGGCCCCTGAGGATCCCTTT
  GTGAAACACTGCAAGCGTTTCTTCGAATTCTGCATCCCCAGACCTATCCTGGTTTTACTCTTATCATTTC
  CATCCATAATGGTCCCACTGGCCCGGATTTTGCCCAATAAGAACCGAGACGAACTGAATGGCTTTTTTAA
  CAAACTCATTAGGAATGTGATTGCCTTGCGGGACCAGCAAGCTGCCGAAGAGAGGCGGAGAGACTTCCTC
  CAAATGGTCCTGGATGCCCGACATTCTGCAAGTCCCATGGGCGTGCAAGACTTTGACATCGTCAGAGACG
  TTTTCTCCTCTACTGGGTGCAAGCCGAACCCTTCCCGGCAACACCAGCCCAGCCCTATGGCCAGGCCTTT
  GACTGTGGATGAGATTGTGGGCCAGGCCTTCATCTTCCTCATCGCTGGCTATGAAATCATCACCAACACA
  CTTTCTTTTGCCACCTACCTACTGGCCACCAACCCTGACTGCCAAGAGAAGCTTCTGAGAGAGGTAGACG
  TTTTTAAGGAGAAACACATGGCCCCTGAGTTCTGCAGCCTCGAGGAAGGCCTGCCCTATCTGGACATGGT
  GATTGCAGAGACGCTGAGGATGTACCCGCCAGCTTTCAGATTCACACGGGAGGCAGCTCAGGACTGCGAG
  GTGCTGGGGCAGCGCATCCCCGCAGGCGCTGTGCTAGAGATGGCCGTGGGTGCCCTGCACCATGACCCTG
  AGCACTGGCCAAGCCCGGAGACCTTCAACCCTGAAAGGTTCACGGCTGAGGCCCGGCAGCAGCACCGGCC
  CTTCACGTACCTGCCCTTCGGGGCCGGCCCACGGAGCTGCCTCGGGGTGCGTCTAGGGCTGCTTGAGGTC
  AAGTTGACACTGCTCCACGTGCTGCACAAGTTCCGGTTCCAAGCCTGCCCTGAGACCCAGGTACCGCTGC
  AGCTAGAATCCAAATCTGCCCTAGGTCCAAAAAATGGTGTCTATATCAAGATCGTATCCCGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001130966
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130966.2, NP_001124438.1
• RefSeq Size: 2385 bp
• RefSeq ORF: 1605 bp
• Locus ID: 6916
• Cytogenetics: 7q34
• Protein Families: Druggable Genome, P450
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to thromboxane A2, a potent vasoconstrictor and inducer of platelet aggregation. The enzyme plays a role in several pathophysiological processes including hemostasis, cardiovascular disease, and stroke. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]'
  Transcript Variant: This variant (3, also known as TXS-III) differs in the 5' UTR, compared to variant 1. Both variants 1 and 3 encode the same isoform (1, also known as isoform TXS-I). CCDS Note: The coding region has been updated to shorten the N-terminus to one that is better supported by available conservation data and peptide data. This CCDS represents use of a downstream start codon, shortening the protein by a single amino acid.