PARN (NM_001134477) Human Untagged Clone

CAT#: SC325208

PARN (untagged)-Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PARN
• Synonyms: DAN; DKCB6; PFBMFT4
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001134477, the custom clone sequence may differ by one or more nucleotides
  ATGGACTTTTTGCTATTTCAGTTTGGCCTTTGCACTTTTAAGTATGACTACACAGATTCA
  AAGTATATAACGAAGTCATTTAACTTCTATGTTTTCCCGAAACCCTTCAATAGATCCTCA
  CCAGATGTCAAATTTGTTTGTCAGAGCTCCAGCATTGACTTTCTAGCAAGCCAGGGATTT
  GATTTTAATAAAGTTTTTCGAAATGGAATTCCATATTTAAATCAGGAAGAAGAAAGACAG
  TTAAGAGAGCAGTATGATGAAAAACGTTCACAGGCGAATGGTGCAGGAGCTCTGTCCTAT
  GTATCTCCTAACACTTCAAAATGTCCTGTCACGATTCCTGAGGATCAAAAGAAGTTTATT
  GACCAAGTGGTAGAGAAAATAGAGGATTTATTACAAAGTGAAGAAAACAAGAACTTGGAT
  TTAGAGCCATGTACCGGGTTCCAAAGAAAACTAATTTATCAGACTTTGAGCTGGAAGTAT
  CCGAAAGGCATTCATGTTGAGACTTTAGAAACTGAAAAGAAGGAGCGATATATAGTTATC
  AGCAAAGTAGATGAAGAAGAACGCAAAAGAAGAGAGCAGCAGAAACATGCCAAAGAACAG
  GAGGAGCTGAATGATGCTGTGGGATTTTCTAGAGTCATTCACGCCATTGCTAATTCGGGA
  AAACTTGTTATTGGACACAATATGCTCTTGGACGTCATGCACACAGTTCATCAGTTCTAC
  TGCCCTCTGCCTGCGGACTTAAGTGAGTTTAAAGAGATGACAACATGTGTTTTCCCCAGA
  CTCTTGGATACTAAATTGATGGCCAGCACACAACCTTTTAAGGATATCATTAACAACACA
  TCCCTTGCGGAATTGGAAAAGCGGTTAAAAGAGACACCTTTCAACCCTCCTAAAGTTGAA
  AGTGCCGAAGGTTTTCCAAGTTATGACACAGCCTCTGAACAACTCCACGAGGCAGGCTAC
  GATGCCTACATCACAGGGCTGTGCTTCATCTCCATGGCCAATTACCTAGGTTCTTTTCTC
  AGCCCTCCAAAAATTCATGTGTCTGCCAGATCAAAACTCATTGAACCTTTTTTTAACAAG
  TTATTTCTTATGAGGGTCATGGATATCCCCTATCTAAACTTGGAAGGACCAGACTTGCAG
  CCTAAACGTGATCATGTTCTCCATGTGACATTCCCCAAAGAATGGAAAACCAGCGACCTT
  TACCAGCTTTTCAGTGCCTTTGGTAACATTCAGATATCCTGGATTGATGACACATCAGCA
  TTTGTTTCCCTTAGCCAGCCCGAGCAAGTAAAGATTGCTGTCAATACCAGCAAATATGCA
  GAAAGCTATCGGATCCAAACCTATGCTGAATATATGGGGAGAAAACAGGAAGAGAAGCAG
  ATCAAAAGAAAGTGGACTGAAGATAGCTGGAAGGAGGCTGACAGCAAACGGTTAAACCCC
  CAGTGCATACCCTACACCCTGCAGAATCACTATTACCGCAACAATAGTTTTACAGCTCCC
  AGCACAGTAGGAAAGAGAAATTTGAGTCCTAGTCAAGAGGAAGCTGGCCTGGAGGACGGA
  GTGTCAGGGGAGATTTCCGACACTGAGCTTGAGCAGACCGATTCCTGTGCAGAGCCCCTC
  TCAGAGGGAAGGAAAAAGGCCAAGAAATTAAAAAGAATGAAGAAGGAGCTTTCTCCAGCA
  GGAAGCATCTCGAAGAACAGCCCTGCCACACTCTTTGAAGTTCCTGACACATGG
  
• Restriction Sites: Please inquire
• ACCN: NM_001134477
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134477.1, NP_001127949.1
• RefSeq Size: 3000 bp
• RefSeq ORF: 3000 bp
• Locus ID: 5073
• Cytogenetics: 16p13.12
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• Gene Summary: 'The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]'
  Transcript Variant: This variant (2) uses an alternate donor splice site in the 5' terminal exon compared to transcript variant 1, resulting in translation initiation from an alternate, in-frame downstream AUG and a shorter isoform (2) compared to isoform 1.