TCF3 / E2A (TCF3) (NM_001136139) Human Untagged Clone

CAT#: SC325258

TCF3 (untagged)-Human transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) (TCF3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCF3
• Synonyms: AGM8; bHLHb21; E2A; E47; ITF1; p75; TCF-3; VDIR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001136139 edited
  CTGAGGTGCCCGCCCTGGCCCCAGGAGAATGAACCAGCCGCAGAGGATGGCGCCTGTGGG
  CACAGACAAGGAGCTCAGTGACCTCCTGGACTTCAGCATGATGTTCCCGCTGCCTGTCAC
  CAACGGGAAGGGCCGGCCCGCCTCCCTGGCCGGGGCGCAGTTCGGAGGTTCAGGTCTTGA
  GGACCGGCCCAGCTCAGGCTCCTGGGGCAGCGGCGACCAGAGCAGCTCCTCCTTTGACCC
  CAGCCGGACCTTCAGCGAGGGCACCCACTTCACTGAGTCGCACAGCAGCCTCTCTTCATC
  CACATTCCTGGGACCGGGACTCGGAGGCAAGAGCGGTGAGCGGGGCGCCTATGCCTCCTT
  CGGGAGAGACGCAGGCGTGGGCGGCCTGACTCAGGCTGGCTTCCTGTCAGGCGAGCTGGC
  CCTCAACAGCCCCGGGCCCCTGTCCCCTTCGGGCATGAAGGGGACCTCCCAGTACTACCC
  CTCCTACTCCGGCAGCTCCCGGCGGAGAGCGGCAGACGGCAGCCTAGACACGCAGCCCAA
  GAAGGTCCGGAAGGTCCCGCCGGGTCTTCCATCCTCGGTGTACCCACCCAGCTCAGGTGA
  GGACTACGGCAGGGATGCCACCGCCTACCCGTCCGCCAAGACCCCCAGCAGCACCTATCC
  CGCCCCCTTCTACGTGGCAGATGGCAGCCTGCACCCCTCAGCCGAGCTCTGGAGTCCCCC
  GGGCCAGGCGGGCTTCGGGCCCATGCTGGGTGGGGGCTCATCCCCGCTGCCCCTCCCGCC
  CGGTAGCGGCCCGGTGGGCAGCAGTGGAAGCAGCAGCACGTTTGGTGGCCTGCACCAGCA
  CGAGCGTATGGGCTACCAGCTGCATGGAGCAGAGGTGAACGGTGGGCTCCCATCTGCATC
  CTCCTTCTCCTCAGCCCCCGGAGCCACGTACGGCGGCGTCTCCAGCCACACGCCGCCTGT
  CAGCGGGGCCGACAGCCTCCTGGGCTCCCGAGGGACCACAGCTGGCAGCTCCGGGGATGC
  CCTCGGCAAAGCACTGGCCTCGATCTACTCCCCGGATCACTCAAGCAATAACTTCTCGTC
  CAGCCCTTCTACCCCCGTGGGCTCCCCCCAGGGCCTGGCAGGAACGTCACAGTGGCCTCG
  AGCAGGAGCCCCCGGTGCCTTATCGCCCAGCTACGACGGGGGTCTCCACGGCCTGCAGAG
  TAAGATAGAAGACCACCTGGACGAGGCCATCCACGTGCTCCGCAGCCACGCCGTGGGCAC
  AGCCGGTGACATGCACACGCTGCTGCCTGGCCACGGGGCGCTGGCCTCAGGTTTCACCGG
  CCCCATGTCACTGGGCGGGCGGCACGCAGGCCTGGTTGGAGGCAGCCACCCCGAGGACGG
  CCTCGCAGGCAGCACCAGCCTCATGCACAACCACGCGGCCCTCCCCAGCCAGCCAGGCAC
  CCTCCCTGACCTGTCTCGGCCTCCCGACTCCTACAGTGGGCTAGGGCGAGCAGGTGCCAC
  GGCGGCCGCCAGCGAGATCAAGCGGGAGGAGAAGGAGGACGAGGAGAACACGTCAGCGGC
  TGACCACTCGGAGGAGGAGAAGAAGGAGCTGAAGGCCCCCCGGGCCCGGACCAGCAGTAC
  GGACGAGGTGCTGTCCCTGGAGGAGAAAGACCTGAGGGACCGGGAGAGGCGCATGGCCAA
  TAACGCGCGGGAGCGGGTGCGCGTGCGGGATATTAACGAGGCCTTCCGGGAGCTGGGGCG
  CATGTGCCAGATGCACCTCAAGTCGGACAAAGCGCAGACCAAGCTGCTCATCCTGCAGCA
  GGCCGTGCAGGTCATCCTGGGGCTGGAGCAGCAGGTGCGAGAGCGGAACCTGAATCCCAA
  AGCAGCCTGTTTGAAACGGCGAGAAGAGGAAAAGGTGTCAGGTGTGGTTGGAGACCCCCA
  GATGGTGCTTTCAGCTCCCCACCCAGGCCTGAGCGAAGCCCACAACCCCGCCGGGCACAT
  GTGAAAGTAAACAAAACCTGAAAGCAAGCAACAAAACATACACTTTGTCAGAGAAGAAAA
  AAATGCCTTAACTATAAAAAGCGGAGAAATGGAAACATATCACTCAAGGGGGATGCTGTG
  GAAACCTG
  
• Restriction Sites: Please inquire
• ACCN: NM_001136139
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001136139.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136139.1, NP_001129611.1
• RefSeq Size: 4091 bp
• RefSeq ORF: 1956 bp
• Locus ID: 6929
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
• Gene Summary: 'This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (2) differs in the 3' UTR, lacks an exon and includes an alternate exon in the 3' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (E47, also known as Pan-1) is shorter than isoform E12. Variants 2 and 4 encode the same isoform (E47). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.