MPPED2 (NM_001145399) Human Untagged Clone

CAT#: SC326546

MPPED2 (untagged)-Human metallophosphoesterase domain containing 2 (MPPED2), transcript variant 2, mRNA

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MPPED2
• Synonyms: 239FB; C11orf8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001145399, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCACATGGGATTCCTTCTCAAGGCAAAGTTACCATAACGGTGGATGAGTACAGCTCAAACCCCACCC
  AGGCATTCACGCACTACAACATCAACCAGAGCAGATTCCAGCCTCCACATGTACATATGGTCGACCCCAT
  CCCATATGACACTCCAAAACCAGCGGGCCACACGCGGTTTGTCTGCATCTCAGACACACACTCCAGAACA
  GATGGTATCCAGATGCCTTATGGGGACATCCTTCTCCACACAGGCGATTTCACCGAGCTGGGACTGCCCT
  CAGAGGTTAAGAAGTTTAATGACTGGTTAGGAAACCTGCCATATGAATATAAAATAGTGATTGCTGGGAA
  TCATGAACTGACATTTGATAAGGAATTCATGGCAGACCTTGTTAAACAGGACTACTACCGTTTCCCCTCT
  GTGTCCAAATTGAAACCAGAGGACTTTGACAATGTTCAGTCCCTCCTGACAAACAGTATTTACTTACAAG
  ATTCGGAGGTAACAGTGAAGGGATTCAGGATATACGGTGCACCTTGGACCCCGTGGTTTAATGGATGGGG
  CTTTAACCTACCCAGAGGTCAGTCTCTGCTGGACAAGTGGAACCTCATCCCTGAGGGCATTGACATACTC
  ATGACACATGGACCTCCTCTAGGTTTTCGAGACTGGGTTCCAAAGGAGCTTCAAAGAGTGGGCTGTGTGG
  AGCTGTTAAACACGGTTCAGAGGCGAGTCCGGCCCAAGCTCCATGTGTTTGGTGGAATCCATGAAGTAAA
  CCCTGTGAGCATCTCCAAGGCACTGAGGACAAAAATATGTTCTCTGCCTTCAAAAACTTCCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145399
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145399.1, NP_001138871.1
• RefSeq Size: 5693 bp
• RefSeq ORF: 834 bp
• Locus ID: 744
• Cytogenetics: 11p14.1
• Gene Summary: 'This gene likely encodes a metallophosphoesterase. The encoded protein may play a role a brain development. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]'
  Transcript Variant: This variant (2) differs in the 5' and the 3' UTR and contains an alternate exon in the 3' coding region compared to variant 1, that results in a frameshift. It encodes isoform 2, which has a shorter and distinct C-terminus compared to isoform 1.