ADAM8 (NM_001164489) Human Untagged Clone

CAT#: SC327203

ADAM8 (untagged)-Human ADAM metallopeptidase domain 8 (ADAM8) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADAM8
• Synonyms: CD156; CD156a; MS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001164489, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGCGGCCTCGGGCTCTGGCTGCTGGGCGCGATGATGCTGCCTGCGATTGCCCCCAGCCGGCCCTGGG
  CCCTCATGGAGCAGTATGAGGTCGTGTTGCCGTGGCGTCTGCCAGGCCCCCGAGTCCGCCGAGCTCTGCC
  CTCCCACTTGGGCCTGCACCCAGAGAGGGTGAGCTACGTCCTTGGGGCCACAGGGCACAACTTCACCCTC
  CACCTGCGGAAGAACAGGGACCTGCTGGGCTCCGGCTACACAGAGACCTATACGGCTGCCAATGGCTCCG
  AGGTGACGGAGCAGCCTCGCGGGCAGGACCACTGCTTCTACCAGGGCCACGTAGAGGGGTACCCGGACTC
  AGCCGCCAGCCTCAGCACCTGTGCCGGCCTCAGGGGTTTCTTCCAGGTGGGGTCAGACCTGCACCTGATC
  GAGCCCCTGGATGAAGGTGGCGAGGGCGGACGGCACGCCGTGTACCAGGCTGAGCACCTGCTGCAGACGG
  CCGGGACCTGCGGGGTCAGCGACGACAGCCTGGGCAGCCTCCTGGGACCCCGGACGGCAGCCGTCTTCAG
  GCCTCGGCCCGGGGACTCTCTGCCATCCCGAGAGACCCGCTACGTGGAGCTGTATGTGGTCGTGGACAAT
  GCAGAGTTCCAGATGCTGGGGAGCGAAGCAGCCGTGCGTCATCGGGTGCTGGAGGTGGTGAATCACGTGG
  ACAAGCTATATCAGAAACTCAACTTCCGTGTGGTCCTGGTGGGCCTGGAGATTTGGAATAGTCAGGACAG
  GTTCCACGTCAGCCCCGACCCCAGTGTCACACTGGAGAACCTCCTGACCTGGCAGGCACGGCAACGGACA
  CGGCGGCACCTGCATGACAACGTACAGCTCATCACGGGTGTCGACTTCACCGGGACTACCGTGGGGTTTG
  CCAGGGTGTCCGCCATGTGCTCCCACAGCTCAGGGGCTGTGAACCAGGACCACAGCAAGAACCCCGTGGG
  CGTGGCCTGTACCATGGCCCATGAGATGGGCCACAACCTGGGCATGGACCATGATGAGAACGTCCAGGGC
  TGCCGCTGCCAGGAACGCTTCGAGGCCGGCCGCTGCATCATGGCGGGCAGCATTGGCTCCAGTTTCCCCA
  GGATGTTCAGTGACTGCAGCCAGGCCTACCTGGAGAGCTTTTTGGAGCGGCCGCAGTCGGTGTGCCTCGC
  CAACGCCCCTGACCTCAGCCACCTGGTGGGCGGCCCCGTGTGTGGGAACCTGTTTGTGGAGCGTGGGGAG
  CAGTGCGACTGCGGCCCCCCCGAGGACTGCCGGAACCGCTGCTGCAACTCTACCACCTGCCAGCTGGCTG
  AGGGGGCCCAGTGTGCGCACGGTACCTGCTGCCAGGAGTGCAAGGTGAAGCCGGCTGGTGAGCTGTGCCG
  TCCCAAGAAGGACATGTGTGACCTCGAGGAGTTCTGTGACGGCCGGCACCCTGAGTGCCCGGAAGACGCC
  TTCCAGGAGAACGGCACGCCCTGCTCCGGGGGCTACTGCTACAACGGGGCCTGTCCCACACTGGCCCAGC
  AGTGCCAGGCCTTCTGGGGGCCAGGTGGGCAGGCTGCCGAGGAGTCCTGCTTCTCCTATGACATCCTACC
  AGGCTGCAAGGCCAGCCGGTACAGGGCTGACATGTGTGGCGTTCTGCAGTGCAAGGGTGGGCAGCAGCCC
  CTGGGGCGTGCCATCTGCATCGTGGATGTGTGCCACGCGCTCACCACAGAGGATGGCACTGCGTATGAAC
  CAGTGCCCGAGGGCACCCGGTGTGGACCAGAGAAGGTTTGCTGGAAAGGACGTTGCCAGGACTTACACGT
  TTACAGATCCAGCAACTGCTCTGCCCAGTGCCACAACCATGGGGTGTGCAACCACAAGCAGGAGTGCCAC
  TGCCACGCGGGCTGGGCCCCGCCCCACTGCGCGAAGCTGCTGACTGAGGTGCACGCAGGCTGCCAGCCGC
  GTGCCGGCCAAGGGCGGGGCTCCAGCCCCATCCAGGGGCCCCCAAGAGCTGGTCCCCACCACCCACCCGG
  GCCAGCCCGCCCGACACCCGGCCTCCTCGGTGGCTCTGAAGAGGCCGCCCCCTGCTCCTCCGGTCACTGT
  GTCCAGCCCACCCTTCCCAGTTCCTGTCTACACCCGGCAGGCACCAAAGCAGGTCATCAAGCCAACGTTC
  GCACCCCCAGTGCCCCCAGTCAAACCCGGGGCTGGTGCGGCCAACCCTGGTCCAGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164489
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164489.1, NP_001157961.1
• RefSeq Size: 3149 bp
• RefSeq ORF: 2229 bp
• Locus ID: 101
• Cytogenetics: 10q26.3
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2009]'
  Transcript Variant: This variant (2) lacks an alternate exon and uses an alternate splice site, which causes a frameshift in the 3' coding region, compared to variant 1. The resulting isoform (2) has a distinct C-terminus and is shorter than isoform 1.