CARD8 (NM_001184903) Human Untagged Clone

CAT#: SC328624

CARD8 (untagged)-Human caspase recruitment domain family member 8 (CARD8) transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CARD8
• Synonyms: CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001184903, the custom clone sequence may differ by one or more nucleotides
  ATGGAAAAAAAGGAGTGTCCAGAAAAGAGTAGCAGCAGTGAGGAAGAGCTGCCGAGACGG
  GACAGTGGATCCAGTAGGAACATAGATGCATCCAAACTCATTAGACTACAAGGATCACGG
  AAACTGTTGGTTGACAATAGCATACGGGAACTGCAATACACAAAAACTGGAATTTTTTTT
  CAGGCTGAGGCCTGTGTGACAAATGATACGGTATACAGGGAGCTACCCTGTGTTTCTGAG
  ACCCTTTGTGACATCTCACATTTTTTCCAAGAAGATGATGAGACAGAGGCAGAGCCATTA
  TTGTTCCGTGCTGTTCCTGAGTGTCAACTATCTGGGGGGGACATTCCCAGTGTATCAGAA
  GAGCAGGAATCTTCAGAGGGACAAGATTCAGGAGACATTTGCTCAGAAGAGAATCAAATA
  GTTTCCTCTTATGCTTCTAAAGTCTGTTTTGAGATCGAAGAAGATTATAAAAATCGTCAG
  TTTCTGGGGCCTGAAGGAAATGTGGATGTTGAGTTGATTGATAAGAGCACAAACAGATAC
  AGCGTTTGGTTCCCCACTGCTGGCTGGTATCTGTGGTCAGCCACAGGCCTCGGCTTCCTG
  GTAAGGGATGAGGTCACAGTGACGATTGCGTTTGGTTCCTGGAGTCAGCACCTGGCCCTG
  GACCTGCAGCACCATGAACAGTGGCTGGTGGGCGGCCCCTTGTTTGATGTCACTGCAGAG
  CCAGAGGAGGCTGTCGCCGAAATCCACCTCCCCCACTTCATCTCCCTCCAAGCAGGTGAG
  GTGGACGTCTCCTGGTTTCTCGTTGCCCATTTTAAGAATGAAGGGATGGTCCTGGAGCAT
  CCAGCCCGGGTGGAGCCTTTCTATGCTGTCCTGGAAAGCCCCAGCTTCTCTCTGATGGGC
  ATCCTGCTGCGGATCGCCAGTGGGACTCGCCTCTCCATCCCCATCACTTCCAACACATTG
  ATCTATTATCACCCCCACCCCGAAGATATTAAGTTCCACTTGTACCTTGTCCCCAGCGAC
  GCCTTGCTAACAAAGGCGATAGATGATGAGGAAGATCGCTTCCATGGTGTGCGCCTGCAG
  ACTTCGCCCCCAATGGAACCCCTGAACTTTGGTTCCAGTTATATTGTGTCTAATTCTGCT
  AACCTGAAAGTAATGCCCAAGTGGATCTCCAGCTTGTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001184903
• ORF Size: 1179 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001184903.1, NP_001171832.1
• RefSeq Size: 5246
• RefSeq ORF: 1179
• Locus ID: 22900
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• Gene Summary: The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
  Transcript Variant: This variant (5) differs in the 5' UTR and lacks an alternate exon in the 3' coding region, which results in a frameshift compared to variant 1. This results in a shorter protein (isoform c), compared to isoform a. Variants 4 and 5 encode the same isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.