PCYT2 (NM_001184917) Human Untagged Clone

CAT#: SC328659

PCYT2 (untagged)-Human phosphate cytidylyltransferase 2 ethanolamine (PCYT2) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCYT2
• Synonyms: ET
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001184917, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATCCGGAACGGGCGCGGGGCTGCAGGCGGCGCAGAGCAGCCGGGCCCGGGGGGCAGGCGCGCCGTGA
  GGGTGTGGTGCGATGGCTGCTATGACATGGTGCATTACGGCCACTCCAACCAGCTGCGCCAGGCACGGGC
  CATGGGTGACTACCTCATCGTAGGCGTGCACACCGATGAGGAGATCGCCAAGCACAAGGGGCCCCCGGTG
  TTCACTCAGGAGGAGAGATACAAGATGGTGCAGGCCATCAAATGGGTGGACGAGGTGGTGCCAGCGGCTC
  CCTACGTCACTACACTAGAGACCCTGGACAAATACAACTGTGACTTCTGTGTTCACGGCAATGACATCAC
  CCTGACTGTAGATGGCCGGGACACCTATGAGGAAGTAAAGCAGGCTGGGAGGTACAGAGAATGCAAGCGC
  ACGCAAGGGGTGTCCACCACAGACCTCGTGGGCCGCATGCTGCTGGTAACCAAAGCCCATCACAGCAGCC
  AGGAGATGTCCTCTGAGTACCGGGAGTATGCAGACAGTTTTGGCAAGCCCCCTCACCCGATACCCGCCGG
  GGACATACTTTCCTCAGAAGGCTGCTCCCAGTGCCCTGGTGGGCGGAACCCCTGGACCGGGGTATCCCAG
  TTCCTGCAGACATCTCAGAAGATCATCCAGTTTGCTTCTGGGAAGGAGCCCCAGCCAGGGGAGACAGTCA
  TCTATGTGGCTGGTGCCTTCGACCTGTTCCACATCGGGCATGTGGACTTCCTGGAGAAGGTGCACAGGCT
  GGCAGAGAGGCCCTACATCATCGCGGGCTTACACTTTGACCAGGAGGTCAATCACTACAAGGGGAAGAAC
  TACCCCATCATGAATCTGCATGAACGGACTCTGAGCGTGCTGGCCTGCCGGTACGTGTCAGAAGTGGTGA
  TTGGAGCCCCGTACGCGGTCACAGCAGAGCTCCTAAGTCACTTCAAGGTGGACCTGGTGTGTCACGGCAA
  GACAGAAATTATCCCTGACAGGGATGGCTCCGACCCATACCAGGAGCCCAAGAGAAGGGGCATCTTCCGT
  CAGATTGACAGTGGCAGCAACCTCACCACAGACCTCATCGTCCAGCGGATCATCACCAACAGGTTGGAGT
  ATGAGGCGCGAAACCAGAAGAAGGAAGCCAAGGAGCTGGCCTTCCTGGAGGCTGCCAGGCAGCAGGCGGC
  ACAGCCCCTGGGGGAGCGCGATGGTGACTTCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001184917
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184917.2, NP_001171846.1
• RefSeq Size: 3295 bp
• RefSeq ORF: 1224 bp
• Locus ID: 5833
• Cytogenetics: 17q25.3
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes an enzyme that catalyzes the formation of CDP-ethanolamine from CTP and phosphoethanolamine in the Kennedy pathway of phospholipid synthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]'
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.