5T4 (TPBG) (NM_001166392) Human Untagged Clone

CAT#: SC328687

TPBG (untagged)-Human trophoblast glycoprotein (TPBG) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TPBG
• Synonyms: 5T4; 5T4AG; M6P1; WAIF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001166392, the custom clone sequence may differ by one or more nucleotides
  ATGCCTGGGGGGTGCTCCCGGGGCCCCGCCGCCGGGGACGGGCGTCTGCGGCTGGCGCGA
  CTAGCGCTGGTACTCCTGGGCTGGGTCTCCTCGTCTTCTCCCACCTCCTCGGCATCCTCC
  TTCTCCTCCTCGGCGCCGTTCCTGGCTTCCGCCGTGTCCGCCCAGCCCCCGCTGCCGGAC
  CAGTGCCCCGCGCTGTGCGAGTGCTCCGAGGCAGCGCGCACAGTCAAGTGCGTTAACCGC
  AATCTGACCGAGGTGCCCACGGACCTGCCCGCCTACGTGCGCAACCTCTTCCTTACCGGC
  AACCAGCTGGCCGTGCTCCCTGCCGGCGCCTTCGCCCGCCGGCCGCCGCTGGCGGAGCTG
  GCCGCGCTCAACCTCAGCGGCAGCCGCCTGGACGAGGTGCGCGCGGGCGCCTTCGAGCAT
  CTGCCCAGCCTGCGCCAGCTCGACCTCAGCCACAACCCACTGGCCGACCTCAGTCCCTTC
  GCTTTCTCGGGCAGCAATGCCAGCGTCTCGGCCCCCAGTCCCCTTGTGGAACTGATCCTG
  AACCACATCGTGCCCCCTGAAGATGAGCGGCAGAACCGGAGCTTCGAGGGCATGGTGGTG
  GCGGCCCTGCTGGCGGGCCGTGCACTGCAGGGGCTCCGCCGCTTGGAGCTGGCCAGCAAC
  CACTTCCTTTACCTGCCGCGGGATGTGCTGGCCCAACTGCCCAGCCTCAGGCACCTGGAC
  TTAAGTAATAATTCGCTGGTGAGCCTGACCTACGTGTCCTTCCGCAACCTGACACATCTA
  GAAAGCCTCCACCTGGAGGACAATGCCCTCAAGGTCCTTCACAATGGCACCCTGGCTGAG
  TTGCAAGGTCTACCCCACATTAGGGTTTTCCTGGACAACAATCCCTGGGTCTGCGACTGC
  CACATGGCAGACATGGTGACCTGGCTCAAGGAAACAGAGGTAGTGCAGGGCAAAGACCGG
  CTCACCTGTGCATATCCGGAAAAAATGAGGAATCGGGTCCTCTTGGAACTCAACAGTGCT
  GACCTGGACTGTGACCCGATTCTTCCCCCATCCCTGCAAACCTCTTATGTCTTCCTGGGT
  ATTGTTTTAGCCCTGATAGGCGCTATTTTCCTCCTGGTTTTGTATTTGAACCGCAAGGGG
  ATAAAAAAGTGGATGCATAACATCAGAGATGCCTGCAGGGATCACATGGAAGGGTATCAT
  TACAGATATGAAATCAATGCGGACCCCAGATTAACGAACCTCAGTTCTAACTCGGATGTC
  TGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001166392
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166392.1, NP_001159864.1
• RefSeq Size: 2881 bp
• RefSeq ORF: 1263 bp
• Locus ID: 7162
• Cytogenetics: 6q14.1
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.