Glucose 6 phosphate isomerase (GPI) (NM_001184722) Human Untagged Clone

CAT#: SC328930

GPI (untagged)-Human glucose-6-phosphate isomerase (GPI) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPI
• Synonyms: AMF; GNPI; NLK; PGI; PHI; SA-36; SA36
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001184722, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTAGCTCTCTGCAGCCTCCAACACCTGGGCTCCAGTGATCCCCGGGCTCTGCCCACCCTCCCCACTG
  CCACTTCCGGGCAGAGGCCAGCAAAGCGGCGGCGCAAGAGTCCCGCCATGGCCGCTCTCACCCGGGACCC
  CCAGTTCCAGAAGCTGCAGCAATGGTACCGCGAGCACCGCTCCGAGCTGAACCTGCGCCGCCTCTTCGAT
  GCCAACAAGGACCGCTTCAACCACTTCAGCTTGACCCTCAACACCAACCATGGGCATATCCTGGTGGATT
  ACTCCAAGAACCTGGTGACGGAGGACGTGATGCGGATGCTGGTGGACTTGGCCAAGTCCAGGGGCGTGGA
  GGCCGCCCGGGAGCGGATGTTCAATGGTGAGAAGATCAACTACACCGAGGGTCGAGCCGTGCTGCACGTG
  GCTCTGCGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCCAGAGGTCAACAAGG
  TTCTGGACAAGATGAAGTCTTTCTGCCAGGGACCCCTCATGGTGACTGAAGCCCTTAAGCCATACTCTTC
  AGGAGGTCCCCGCGTCTGGTATGTCTCCAACATTGATGGAACTCACATTGCCAAAACCCTGGCCCAGCTG
  AACCCCGAGTCCTCCCTGTTCATCATTGCCTCCAAGACCTTTACTACCCAGGAGACCATCACGAATGCAG
  AGACGGCGAAGGAGTGGTTTCTCCAGGCGGCCAAGGATCCTTCTGCAGTGGCGAAGCACTTTGTTGCCCT
  GTCTACTAACACAACCAAAGTGAAGGAGTTTGGAATTGACCCTCAAAACATGTTCGAGTTCTGGGATTGG
  GTGGGAGGACGCTACTCGCTGTGGTCGGCCATCGGACTCTCCATTGCCCTGCACGTGGGTTTTGACAACT
  TCGAGCAGCTGCTCTCGGGGGCTCACTGGATGGACCAGCACTTCCGCACGACGCCCCTGGAGAAGAACGC
  CCCCGTCTTGCTGGCCCTGCTGGGTATCTGGTACATCAACTGCTTTGGGTGTGAGACACACGCCATGCTG
  CCCTATGACCAGTACCTGCACCGCTTTGCTGCGTACTTCCAGCAGGGCGACATGGAGTCCAATGGGAAAT
  ACATCACCAAATCTGGAACCCGTGTGGACCACCAGACAGGCCCCATTGTGTGGGGGGAGCCAGGGACCAA
  TGGCCAGCATGCTTTTTACCAGCTCATCCACCAAGGCACCAAGATGATACCCTGTGACTTCCTCATCCCG
  GTCCAGACCCAGCACCCCATACGGAAGGGTCTGCATCACAAGATCCTCCTGGCCAACTTCTTGGCCCAGA
  CAGAGGCCCTGATGAGGGGAAAATCGACGGAGGAGGCCCGAAAGGAGCTCCAGGCTGCGGGCAAGAGTCC
  AGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCTTTGAAGGAAATCGCCCAACCAACTCTATTGTGTTC
  ACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCATGTATGAGCACAAGATCTTCGTTCAGGGCA
  TCATCTGGGACATCAACAGCTTTGACCAGTGGGGAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGA
  GCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATC
  AAGCAGCAGCGCGAGGCCAGAGTCCAATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001184722
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184722.1, NP_001171651.1
• RefSeq Size: 4275 bp
• RefSeq ORF: 1710 bp
• Locus ID: 2821
• Cytogenetics: 19q13.11
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway, Starch and sucrose metabolism
• Gene Summary: 'This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]'
  Transcript Variant: This variant (1) lacks an in-frame exon in the central coding region, compared to variant 3. The encoded isoform (1) is shorter, compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.