NBL1 (NM_001278164) Human Untagged Clone

CAT#: SC334219

NBL1 (untagged) - Human neuroblastoma 1, DAN family BMP antagonist (NBL1), transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NBL1
• Synonyms: D1S1733E; DAN; DAND1; NB; NO3
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC334219 representing NM_001278164.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGATGCTTCGGGTCCTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAAC
  AAGCTGGCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGCCAC
  AGCGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGCTACAGCGTCCCC
  AACACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGCATGCCAGCCCAGTCCATGTGG
  GAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTGCCCAGGGTGGACAAGCTGGTGGAGAAGATC
  CTGCACTGTAGCTGCCAGGCCTGCGGCAAGGAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGC
  GAGGACGGGCCGGGATCCCAGCCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAG
  ACCCCTGAGCCCGAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGACTGA
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001278164
• Insert Size: 546 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278164.1
• RefSeq Size: 2060 bp
• RefSeq ORF: 546 bp
• Locus ID: 4681
• UniProt ID: P41271
• Cytogenetics: 1p36.13
• Protein Families: Secreted Protein
• MW: 19.4 kDa
• Gene Summary: This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013]
  Transcript Variant: This variant (6) uses an alternate splice site in the 5' exon, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2, 3, 4, 6, 7, and 8 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.