PRTFDC1 (NM_001282786) Human Untagged Clone

CAT#: SC334321

PRTFDC1 (untagged) - Human phosphoribosyl transferase domain containing 1 (PRTFDC1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRTFDC1
• Synonyms: HHGP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC334321 representing NM_001282786.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCGGGAGCAGCGAGGAGGCGCCAGACTACGGGCGAGGCGTCGTGATTATGGATGATTGGCCAGGG
  TATGACTTGAATTTATTCACGTACCCACAGCACTATTATGGAGACTTGGAGTATGTCCTCATCCCTCAT
  GGTATCATTGTGGACAGAATTGAGCGGCTGGCCAAGGATATTATGAAAGACATAGGATATAGTGACATC
  ATGGTCCTGTGTGTGCTTAAAGGAGGTTACAAATTCTGTGCTGATCTCGTAGAACACCTTAAGAACATC
  AGCCGAAATTCAGATCGATTTGTCTCAATGAAGGTTGATTTCATCAGACTAAAAAGTTACAGGAATGAC
  CAGTCCATGGGTGAGATGCAGATAATCGGAGGCGATGATCTTTCAACGCTGGCTGGAAAGAATGTTCTC
  ATTGTTGAGGATGTTGTCGGAACTGGGAGGACCATGAAAGCACTACTCAGCAATATAGAGAAATACAAG
  CCCAACATGATTAAGGTAGCCAGTTTGTTGGTGAAGAGAACATCCAGAAGTGACGGCTTTAGACCTGAC
  TCACATATGCGTCATCAATGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001282786
• Insert Size: 573 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282786.1
• RefSeq Size: 1925 bp
• RefSeq ORF: 573 bp
• Locus ID: 56952
• UniProt ID: Q9NRG1
• Cytogenetics: 10p12.1
• Protein Families: Druggable Genome
• MW: 21.6 kDa
• Gene Summary: Has low, barely detectable phosphoribosyltransferase activity (in vitro). Binds GMP, IMP and alpha-D-5-phosphoribosyl 1-pyrophosphate (PRPP). Is not expected to contribute to purine metabolism or GMP salvage.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1.