TMEM150B (NM_001282011) Human Untagged Clone

CAT#: SC334672

TMEM150B (untagged) - Human transmembrane protein 150B (TMEM150B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TMEM150B
• Synonyms: DRAM3; TMEM224; TTN2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC334672 representing NM_001282011.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTGGGGCTACCTGTCGCTGATGCCTGTCTTCCTAGCTGTCTGGGCTATCTCTGGCGTCTGGATCGTT
  TTTGCCATTGCAGTGACCAACAGGACTGTGGACCTCAGTAAAGGCTTTCCCTACATCAGCATCTGCGGA
  TCCTTCCCCCCTCAGAGCTGCATCTTCAGCCAGGTGCTCAATATGGGAGCTGCTCTGGCCGCGTGGATC
  TGCATTGTCCGTTACCACCAGCTCCGGGACTGGGGCGTCAGAAGGTGGCCTAACCAGCTGATCCTATGG
  ACGGGTCTTCTGTGTGCCCTGGGCACCTCCGTGGTAGGCAATTTCCAGGAAAAGAACCAGCGGCCTACG
  CACTTGGCAGGGGCCTTCCTTGCCTTCATCTTGGGTAACGTCTACTTCTGGCTGCAGCTCCTCCTGTGG
  AGGCTGAAGAGGCTGCCCCAGCCCGGGGCTGCCTGGATTGGGCCCCTCCGCCTGGGCCTCTGCAGCGTC
  TGCACCATCCTCATTGTGGCCATGATCGTCCTCCACGCCTGCTCGCTGCGTAGCGTCTCTGCGGCCTGC
  GAGTGGGTCGTGGCCATGCTGCTGTTCGCGCTCTTCGGTCTCTTAGCCGTTGACTTCTCCGCCCTGGAG
  AGCTGCACCCTGTGTGTTCAGCCGTGGCCCAGCCTCAGCCCCCCGCCGGCCTCCCCCATCTCCCTGCCG
  GTCCAGCTGTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001282011
• Insert Size: 702 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282011.1
• RefSeq Size: 982 bp
• RefSeq ORF: 702 bp
• Locus ID: 284417
• UniProt ID: A6NC51
• Cytogenetics: 19q13.42
• Protein Families: Transmembrane
• MW: 25.7 kDa
• Gene Summary: This gene encodes a protein that belongs to the DRAM (damage-regulated autophagy modulator) family of membrane-spanning proteins. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein (isoform a).