MAST4 (NM_001290228) Human Untagged Clone

CAT#: SC334720

MAST4 (untagged) - Human microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAST4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC334720 representing NM_001290228.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGGGAGAAAGTTTCGGAGGCGCCAGAGCCGGTGCCCCGCGGCTGCAGTGGCCACGGCAGCCGGACT
  CCAGCCTCTGCGCTGGTCGCCGCGTCCTCTCCGGGTGCTTCCTCGGCCGAGTCCTCCTCGGGCTCAGAA
  ACTCTGTCGGAGGAAGGGGAGCCCGGCGGCTTCTCCAGAGAGCATCAGCCGCCGCCGCCGCCGCCGTTG
  GGAGGCACCCTGGGCGCCCGGGCGCCCGCCGCGTGGGCTCCGGCAAGCGTGCTGCTGGAGCGCGGAGTC
  CTTGCGCTGCCGCCGCCGCTTCCCGGAGGAGCTGTGCCGCCCGCGCCCCGGGGCAGCAGCGCGTCCCAG
  GAGGAGCAGGACGAGGAGCTTGACCACATATTATCCCCTCCACCCATGCCGTTTCGGAAATGCAGCAAC
  CCAGATGTGGCTTCTGGCCCTGGAAAATCACTGAAGTATAAAAGACAGCTGAGTGAGGATGGAAGACAG
  CTAAGGCGAGGGAGCCTGGGAGGAGCCCTGACTGGGAGGTACCTTCTTCCAAACCCGGTGGCGGGACAG
  GCCTGGCCGGCCTCTGCAGAGACGTCCAACCTCGTGCGCATGCGCAGCCAGGCCCTGGGCCAGTCGGCG
  CCCTCGCTCACCGCCAGCCTGAAGGAGCTGAGTCTCCCCAGAAGAGGAAGTTTCCCTGTGTGTCCAAAT
  GCTGGGAGAACATCACCCCTTGGATGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001290228
• Insert Size: 717 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290228.1
• RefSeq Size: 1062 bp
• RefSeq ORF: 717 bp
• Locus ID: 375449
• UniProt ID: O15021
• Cytogenetics: 5q12.3
• Protein Families: Druggable Genome, Protein Kinase
• MW: 24.3 kDa
• Gene Summary: This gene encodes a member of the microtubule-associated serine/threonine protein kinases. The proteins in this family contain a domain that gives the kinase the ability to determine its own scaffold to control the effects of their kinase activities. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]
  Transcript Variant: This variant (6) uses a distinct 3' coding region and 3' UTR compared to variant 3. The encoded protein (isoform f) has a shorter and distinct C-terminus compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.