MBD3 (NM_001281454) Human Untagged Clone
CAT#: SC334891
MBD3 (untagged) - Human methyl-CpG binding domain protein 3 (MBD3), transcript variant 2
"NM_001281454" in other vectors (1)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | MBD3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC334891 representing NM_001281454.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCGGAAGAGCCCGAGCGGGAAGAAGTTCCGCAGCAAGCCGCAGCTGGCGCGCTACCTGGGCGGC TCCATGGACCTGAGCACCTTCGACTTCCGCACGGGCAAGATGCTGATGAGCAAGATGAACAAGAGCCGC CAGCGCGTGCGCTACGACTCCTCCAACCAGGTCAAGGGCAAGCCCGACCTGAACACGGCGCTGCCCGTG CGCCAGACGGCGTCCATCTTCAAGCAGCCGGTGACCAAGATTACCAACCACCCCAGCAACAAGGTCAAG AGCGACCCGCAGAAGGCGGTGGACCAGCCGCGCCAGCTCTTCTGGGAGAAGAAGCTGAGCGGCCTGAAC GCCTTCGACATTGCTGAGGAGCTGGTCAAGACCATGGACCTCCCCAAGGGCCTGCAGGGGGTGGGACCT GGCTGCACGGATGAGACGCTGCTGTCGGCCATCGCCAGCGCCCTGCACACTAGCACCATGCCCATCACG GGACAGCTCTCGGCCGCCGTGGAGAAGAACCCCGGCGTATGGCTCAACACCACGCAGCCCCTGTGCAAA GCCTTCATGGTGACCGACGAGGACATCAGGAAGCAGGAAGAGCTGGTGCAGCAGGTGCGGAAGCGGCTG GAGGAGGCGCTGATGGCCGACATGCTGGCGCACGTGGAGGAGCTGGCCCGTGACGGGGAGGCGCCGCTG GACAAGGCCTGCGCTGAGGACGACGACGAGGAAGACGAGGAGGAGGAGGAGGAGGAGCCCGACCCGGAC CCGGAGATGGAGCACGTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites |
SgfI-MluI
Plasmid Map
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ACCN | NM_001281454 |
Insert Size | 780 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001281454.1 |
RefSeq Size | 2417 bp |
RefSeq ORF | 780 bp |
Locus ID | 53615 |
UniProt ID | O95983 |
Cytogenetics | 19p13.3 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 29 kDa |
Gene Summary | DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter, compared to isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC236997 | MBD3 (myc-DDK-tagged) - Human methyl-CpG binding domain protein 3 (MBD3), transcript variant 2 |
USD 420.00 |
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