KCTD20 (NM_001286580) Human Untagged Clone

CAT#: SC334982

KCTD20 (untagged) - Human potassium channel tetramerization domain containing 20 (KCTD20), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCTD20
• Synonyms: C6orf69; dJ108K11.3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC334982 representing NM_001286580.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTTGGACCAGGAAGAGAGTACAACTTCACTCGGCCCAATGAGAAGGGAGAGTATGAGATTGCTGAA
  GGCATCAGTGCAACTGTATTTCGCACAGTGCTGGATTATTACAAAACCGGTATCATCAATTGTCCTGAT
  GGCATCTCTATCCCAGATCTTAGAGATACTTGTGATTATCTCTGCATTAATTTTGACTTCAACACTATC
  CGATGTCAAGATCTGAGTGCTTTACTCCATGAACTGTCTAATGACGGTGCTCATAAGCAGTTTGATCAC
  TACCTCGAAGAGCTCATCTTGCCCATCATGGTGGGCTGTGCCAAGAAAGGAGAACGAGAGTGCCACATT
  GTTGTGCTGACGGATGAGGATTCTGTGGACTGGGATGAAGACCACCCTCCACCAATGGGGGAGGAATAT
  TCCCAAATTCTTTATAGCTCCAAGCTCTACAGATTCTTCAAATATATTGAGAATAGGGATGTTGCAAAA
  ACAGTGTTAAAGGAACGGGGCCTAAAAAACATTCGCATTGGAATTGAAGGTTACCCTACCTGTAAAGAA
  AAAATTAAGAGAAGGCCTGGCGGCCGGTCTGAAGTCATCTATAATTATGTACAACGCCCCTTCATCCAG
  ATGTCATGGGAAAAGGAAGAAGGGAAGAGTCGCCATGTGGATTTCCAGTGTGTTCGAAGCAAATCCCTC
  ACGAATCTGGTAGCTGCTGGAGATGATGTCTTGGAGGACCAGGAGATATTAATGCATCACCCACCCCAA
  GTGGATGAACTTGACCGGCTAAATGCCCCACTTTCTCAGATGGCTTCTAACGACTTTCAGGATTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001286580
• Insert Size: 825 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286580.1
• RefSeq Size: 5369 bp
• RefSeq ORF: 825 bp
• Locus ID: 222658
• UniProt ID: Q7Z5Y7
• Cytogenetics: 6p21.31
• Protein Families: Druggable Genome, Ion Channels: Other
• MW: 31.7 kDa
• Gene Summary: Promotes the phosphorylation of AKT family members.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate exon, resulting in translation initiation at at alternate start codon and a frameshifted 5' coding region, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is shorter than isoform a.