ALP (PDLIM3) (NM_001257962) Human Untagged Clone

CAT#: SC335000

PDLIM3 (untagged) - Human PDZ and LIM domain 3 (PDLIM3), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDLIM3
• Synonyms: ALP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335000 representing NM_001257962.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCCCAGACGGTGATCCTCCCGGGCCCTGCGCCCTGGGGCTTCAGGCTCTCAGGGGGCATAGACTTC
  AACCAGCCTTTGGTCATCACCAGGATTACACCAGGAAGCAAGGCGGCAGCTGCCAACCTGTGTCCTGGA
  GATGTCATCCTGGCTATTGACGGCTTTGGGACAGAGTCCATGACTCATGCTGATGCGCAGGACAGGATT
  AAAGCAGCAGCTCACCAGCTGTGTCTCAAAATTGACAGGGGAGAAACTCACTTATGGTCTCCACAAGTA
  TCTGAAGATGGGAAAGCCCATCCTTTCAAAATCAACTTAGAATCAGAACCACAGGACGGGAACTACTTT
  GAACACAAGCATAATATTCGGCCCAAACCTTTCGTGATCCCGGGCCGAAGCAGCGAGCCCACAGCCTCG
  GTGCCCCCCGAGTCGGACGTGTACCGGATGCTCCACGACAATCGGAATGAGCCCACACAGCCTCGCCAG
  TCGGGCTCCTTCAGAGTGCTCCAGGGAATGGTGGACGATGGCTCTGATGACCGTCCGGCTGGAACGCGG
  AGTGTGAGAGCTCCGGTGACGAAAGTCCATGGCGGTTCAGGCGGGGCACAGAGGATGCCGCTCTGTGAC
  AAATGTGGGAGTGGCATAGTCGGTGCTGTGGTGAAGGCGCGGGATAAGTACCGGCACCCTGAGTGCTTC
  GTGTGTGCCGACTGCAACCTCAACCTCAAGCAAAAGGGCTACTTCTTCATAGAAGGGGAGCTGTACTGC
  GAAACCCACGCAAGAGCCCGCACAAAGCCCCCAGAGGGCTATGACACGGTCACTCTGTATCCCAAAGCT
  TAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001257962
• Insert Size: 831 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001257962.1
• RefSeq Size: 2589 bp
• RefSeq ORF: 831 bp
• Locus ID: 27295
• Cytogenetics: 4q35.1
• MW: 30.2 kDa
• Gene Summary: The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Aberrant alternative splicing of this gene may play a role in myotonic dystrophy. [provided by RefSeq, Apr 2012]
  Transcript Variant: This variant (3) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.