GANC (NM_001301410) Human Untagged Clone

CAT#: SC335051

GANC (untagged) - Human glucosidase, alpha, neutral C (GANC), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GANC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335051 representing NM_001301410.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAAGCAGCAGTGAAAGAGGAAATAAGTCTTGAAGATGAAGCTGTAGATAAAAACATTTTCAGAGAC
  TGTAACAAGATCGCATTTTACAGGCGTCAGAAACAGTGGCTTTCCAAGAAGTCCACCTATCAGGCATTA
  TTGGATTCAGTCACAACAGATGAAGACAGCACCAGGTTCCAAATCATCAATGAAGCAAGTAAGGTCAGG
  TGCCAGGACCAGAGCCCAACAACTCTGGATGCCTTCAAAGGCAAAGAAACCACAGTCCAGGAGGAGACA
  CAGGATCTCAGCACAAGACAGCACCTGGCTCAAAAGGAAGCTGAGGAGCCTGATAAACAAGGACAAATT
  ACCTGGGACATCAGCAACGTAGCTCCCAAGACTGAGAACTTTGAGGACCTCTTGGAACAAACTCCATGC
  TCAGCCTGTTTGCAAGGTTTCACCAGAGACAACACTTCCAGCAGCTTCTCCGAGGATGCAGGAATATCT
  CTGAGCACCTCTGTGAAGTTACCCCTTGCCTCTTCATCTAAGCCTAATGAAAATGAAGCTTTGCCCAAC
  CAGCCACCTTCTCAGCAGATAAGGCACAACTACCGCTTATTAGCCAGTACTGTGTCTGCAGAAATGGCC
  AGGAACAGGCAGCTAATTTGGGAACTGGCCAGACAATCAGAAGCACACCTGGACAGGCCAGTTGTCCTT
  GGGGAACAGGCCAGTGCCCAGCGTGAGGTTGCCAGTATTCTTTGTAGAAAATCAGTGCTTGCAATAAAC
  CAACTGGCTGCAACTGTAGAAGGGACAATTAGTGTCCTACAGCAATTTAATGAACTATTAGACCATCCT
  CTGGATCCCGGGAGGTCTTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001301410
• Insert Size: 849 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301410.1
• RefSeq Size: 1559 bp
• RefSeq ORF: 849 bp
• Locus ID: 2595
• Cytogenetics: 15q15.1
• Protein Families: Druggable Genome
• Protein Pathways: Galactose metabolism, Metabolic pathways, Starch and sucrose metabolism
• MW: 31.6 kDa
• Gene Summary: Glycosyl hydrolase enzymes hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. This gene encodes a member of glycosyl hydrolases family 31. This enzyme hydrolyses terminal, non-reducing 1,4-linked alpha-D-glucose residues and releases alpha-D-glucose. This is a key enzyme in glycogen metabolism and its gene localizes to a chromosomal region (15q15) that is associated with susceptibility to diabetes. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2014]
  Transcript Variant: This variant (3) lacks several exons in the 3' coding region, and contains an alternate 3' structure, compared to variant 1. It encodes isoform 3 which is shorter, and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.