PCYT2 (NM_001256434) Human Untagged Clone

CAT#: SC335518

PCYT2 (untagged) - Human phosphate cytidylyltransferase 2, ethanolamine (PCYT2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCYT2
• Synonyms: ET
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001256434, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGATCGCCAAGCACAAGGGGCCCCCGGTGTTCACTCAGGAGGAGAGATACAAGATGGTGCAGGCCA
  TCAAATGGGTGGACGAGGTGGTGCCAGCGGCTCCCTACGTCACTACACTAGAGACCCTGGACAAATACAA
  CTGTGACTTCTGTGTTCACGGCAATGACATCACCCTGACTGTAGATGGCCGGGACACCTATGAGGAAGTA
  AAGCAGGCTGGGAGGTACAGAGAATGCAAGCGCACGCAAGGGGTGTCCACCACAGACCTCGTGGGCCGCA
  TGCTGCTGGTAACCAAAGCCCATCACAGCAGCCAGGAGATGTCCTCTGAGTACCGGGAGTATGCAGACAG
  TTTTGGCAAGCCCCCTCACCCGATACCCGCCGGGGACATACTTTCCTCAGAAGGCTGCTCCCAGTGCCCT
  GGTGGGCGGAACCCCTGGACCGGGGTATCCCAGTTCCTGCAGACATCTCAGAAGATCATCCAGTTTGCTT
  CTGGGAAGGAGCCCCAGCCAGGGGAGACAGTCATCTATGTGGCTGGTGCCTTCGACCTGTTCCACATCGG
  GCATGTGGACTTCCTGGAGAAGGTGCACAGGCTGGCAGAGAGGCCCTACATCATCGCGGGCTTACACTTT
  GACCAGGAGGTCAATCACTACAAGGGGAAGAACTACCCCATCATGAATCTGCATGAACGGACTCTGAGCG
  TGCTGGCCTGCCGGTACGTGTCAGAAGTGGTGATTGGAGCCCCGTACGCGGTCACAGCAGAGCTCCTAAG
  TCACTTCAAGGTGGACCTGGTGTGTCACGGCAAGACAGAAATTATCCCTGACAGGGATGGCTCCGACCCA
  TACCAGGAGCCCAAGAGAAGGGGCATCTTCCGTCAGATTGACAGTGGCAGCAACCTCACCACAGACCTCA
  TCGTCCAGCGGATCATCACCAACAGGTTGGAGTATGAGGCGCGAAACCAGAAGAAGGAAGCCAAGGAGCT
  GGCCTTCCTGGAGGCTGCCAGGCAGCAGGCGGCACAGCCCCTGGGGGAGCGCGATGGTGACTTCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256434
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256434.2, NP_001243363.1
• RefSeq Size: 3293 bp
• RefSeq ORF: 1047 bp
• Locus ID: 5833
• Cytogenetics: 17q25.3
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes an enzyme that catalyzes the formation of CDP-ethanolamine from CTP and phosphoethanolamine in the Kennedy pathway of phospholipid synthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]'
  Transcript Variant: This variant (3) uses an alternate splice site in its 5' coding region and uses an alternate downstream start codon, compared to variant 1. The encoded isoform (3) has a distinct and shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.