ENT2 (SLC29A2) (NM_001300869) Human Untagged Clone

CAT#: SC335616

SLC29A2 (untagged) - Human solute carrier family 29 (equilibrative nucleoside transporter), member 2 (SLC29A2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC29A2
• Synonyms: DER12; ENT2; HNP36
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001300869, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGCGAGGAGACGCCCCGCGGGACAGCTACCACCTGGTCGGGATCAGCTTCTTCATCCTGGGGCTGG
  GCACCCTCCTTCCCTGGAACTTCTTCATCACCGCCATCCCGTACTTCCAGGCGCGACTGGCCGGGGCCGG
  CAACAGCACAGCCAGGATCCTGAGCACCAACCACACGGGTCCCGAGGATGCCTTCAACTTCAACAATTGG
  GTGACGCTGCTGTCCCAGCTGCCCCTGCTGCTCTTCACCCTCCTCAACTCCTTCCTGTACCAGTGCGTCC
  CGGAGACGGTGCGCATTCTGGGCAGCCTGCTGGCCATACTGCTGCTCTTTGCCCTGACAGCAGCGCTGGT
  CAAGGTGGACATGAGCCCCGGACCCTTCTTCTCCATCACCATGGCCTCCGTCTGCTTCATCAACTCCTTC
  AGTGCAGTCCTACAGGGCAGCCTCTTCGGGCAGCTGGGCACCATGCCCTCCACCTACAGCACCCTCTTCC
  TCAGCGGCCAGGGCCTGGCTGGGATCTTTGCTGCCCTTGCCATGCTCCTGTCCATGGCCAGTGGCGTGGA
  CGCCGAGACCTCTGCCCTGGGGTACTTTATCACGCCCTGTGTGGGCATCCTCATGTCCATCGTGTGTTAC
  CTGAGCCTGCCTCACCTGAAGTTTGCCCGCTACTACCTGGCCAATAAATCATCCCAGGCCCAAGCTCAGG
  AGCTGGAGACCAAAGCTGAGCTCCTCCAGTCTGATCTGGCTGACAGCGCTGTGCCTTGTGTTGGTCTTCA
  CAGTCACCCTGTCCGTCTTCCCCGCCATCACAGCCATGGTGACCAGCTCCACCAGTCCTGGGAAGTGGAG
  TCAGTTCTTCAACCCCATCTGCTGCTTCCTCCTCTTCAACATCATGGACTGGCTGGGACGGAGCCTGACC
  TCTTACTTCCTGTGGCCAGACGAGGACAGCCGGCTGCTGCCCCTGCTGGTCTGCCTGCGGTTCCTGTTCG
  TGCCCCTCTTCATGCTGTGCCACGTGCCCCAGAGGTCCCGGCTGCCCATCCTCTTCCCACAGGATGCCTA
  CTTCATCACCTTCATGCTGCTCTTTGCCGTTTCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001300869
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001300869.1, NP_001287798.1
• RefSeq Size: 2561 bp
• RefSeq ORF: 1086 bp
• Locus ID: 3177
• Cytogenetics: 11q13.2
• Protein Families: Transmembrane
• Gene Summary: 'The uptake of nucleosides by transporters, such as SLC29A2, is essential for nucleotide synthesis by salvage pathways in cells that lack de novo biosynthetic pathways. Nucleoside transport also plays a key role in the regulation of many physiologic processes through its effect on adenosine concentration at the cell surface (Griffiths et al., 1997 [PubMed 9396714]).[supplied by OMIM, Nov 2008]'
  Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate exon compared to variant 1, that causes a frameshift. The resulting isoform (b) has a shorter and distinct C-terminus compared to isoform a.