Glucose 6 phosphate isomerase (GPI) (NM_001289790) Human Untagged Clone

CAT#: SC336632

GPI (untagged) - Human glucose-6-phosphate isomerase (GPI), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPI
• Synonyms: AMF; GNPI; NLK; PGI; PHI; SA-36; SA36
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001289790, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGCTCTCACCCGGGACCCCCAGTTCCAGAAGCTGCAGCAATGGTACCGCGAGCACCGCTCCGAGC
  TGAACCTGCGCCGCCTCTTCGATGCCAACAAGGACCGCTTCAACCACTTCAGCTTGACCCTCAACACCAA
  CCATGGGCATATCCTGGTGGATTACTCCAAGAACCTGGTGACGGAGGACGTGATGCGGATGCTGGTGGAC
  TTGGCCAAGTCCAGGGGCGTGGAGGCCGCCCGGGAGCGGATGTTCAATGGTGAGAAGATCAACTACACCG
  AGGGTCGAGCCGTGCTGCACGTGGCTCTGCGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGA
  TGTGATGCCAGAGGTCAACAAGGTTCTGGACAAGATGAAGTCTTTCTGCCAGGGACCCCTCATGGTGACT
  GAAGCCCTTAAGCCATACTCTTCAGGAGGTCCCCGCGTCTGGTATGTCTCCAACATTGATGGAACTCACA
  TTGCCAAAACCCTGGCCCAGCTGAACCCCGAGTCCTCCCTGTTCATCATTGCCTCCAAGACCTTTACTAC
  CCAGGAGACCATCACGAATGCAGAGACGGCGAAGGAGTGGTTTCTCCAGGCGGCCAAGGATCCTTCTGCA
  GTGGCGAAGCACTTTGTTGCCCTGTCTACTAACACAACCAAAGTGAAGGAGTTTGGAATTGACCCTCAAA
  ACATGTTCGAGTTCTGGGATTGGGTGGGAGGACGCTACTCGCTGTGGTCGGCCATCGGACTCTCCATTGC
  CCTGCACGTGGGTTTTGACAACTTCGAGCAGCTGCTCTCGGGGGCTCACTGGATGGACCAGCACTTCCGC
  ACGACGCCCCTGGAGAAGAACGCCCCCGTCTTGCTGGCCCTGCTGGGTATCTGGTACATCAACTGCTTTG
  GGTGTGAGACACACGCCATGCTGCCCTATGACCAGTACCTGCACCGCTTTGCTGCGTACTTCCAGCAGGG
  CGACATGGAGTCCAATGGGAAATACATCACCAAATCTGGAACCCGTGTGGACCACCAGACAGGCCCCATT
  GTGTGGGGGGAGCCAGGGACCAATGGCCAGCATGCTTTTTACCAGCTCATCCACCAAGGCACCAAGATGA
  TACCCTGTGACTTCCTCATCCCGGTCCAGACCCAGCACCCCATACGGAAGGGTCTGCATCACAAGATCCT
  CCTGGCCAACTTCTTGGCCCAGACAGAGGCCCTGATGAGGGGAAAATCGACGGAGGAGGCCCGAAAGGAG
  CTCCAGGCTGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCTTTGAAGGAAATC
  GCCCAACCAACTCTATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCATGTATGA
  GCACAAGATCTTCGTTCAGGGCATCATCTGGGACATCAACAGCTTTGACCAGTGGGGAGTGGAGCTGGGA
  AAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTA
  CCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289790
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289790.2, NP_001276719.1
• RefSeq Size: 4132 bp
• RefSeq ORF: 1593 bp
• Locus ID: 2821
• Cytogenetics: 19q13.11
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway, Starch and sucrose metabolism
• Gene Summary: 'This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]'
  Transcript Variant: This variant (4) differs in the 5' UTR, uses a downstream start codon, and lacks an in-frame exon in the central coding region, compared to variant 3. The encoded isoform (4) is shorter, compared to isoform 3.