VP1 (NC_012042) Virus Tagged ORF Clone

CAT#: VC100546

Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human bocavirus 2], codon optimized for human cell expression, YP_002586775

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100546 represents NCBI reference of YP_002586775 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCCTATCAAGAGACAGCCGGGTGGCTGGGTGTTGCCGGGGTATAAATACCTGGGACCTTTCAACC
  CCCTCGAAAATGGCGAACCGGTGAACAAGGCTGATAGAGCAGCCCAAGCCCATGATAAGTCTTACAGTGA
  AATAATAAAGTCAGGTAAGAATCCCTACCTGTATTTTAACAAGGCAGACGAGAAGTTCATTGATGACCTG
  AAAAACGATTGGTCACTGGGGGGCATCATTGGCAGTAGTTTCTTTAAACTGAAGAGAGCTGTCGCTCCAG
  CCCTGGGCAACAAAGAGAGGGCACAGAAGAGACATTTTTACTTTGCCAATTCCAACAAGGGGGCTAAGAA
  ACCAAAGAACAATGAGCCCAAACCAGGCACCTCTAAGATGAGTGAGAATGAAATCCAGGACCAACAACCC
  TCTGGTAGCATGGAAGAAAGAGGCGGCGGCGGCGGAGCAGTCGGCTCCGTAGGCGGGGGCAAGGGTTCAA
  GTGTCGGCATCAGCACTGGAGGGTGGGTCGGAGGGTCTTATTTTACTGACTCCTACGTGATCACCAAGAA
  CACACGGCAATTCTTGGTCAAGATTCAGAACGATCATAAGTATAGAACAGAGAATATCATCCCTAGCAAC
  GCAGGAGGGAAGTCACAGCGATGTGTGAGCACCCCATGGAGTTACTTCAATTTTAACCAGTACAGTTCCC
  ACTTCAGTCCCCAGGATTGGCAGCATTTGACTAACGAGTATAAACGCTTTAAACCAAGAAAAATGCATGT
  GAAAATTTACAATCTGCAGATTAAACAGATTCTCAGCAATGGAGCCGATACAACATATAATAATGATCTT
  ACCGCAGGAGTACATATTTTCTGTGATGGCGAGCACGCTTACCCAAACGCAACTCACCCCTGGGACGAGG
  ACGTGATGCCAGAACTTCCCTACGAAACCTGGTACCTGTTTCAGTATGGCTACATTCCAGTTATCCACGA
  ACTGGCTGAAATGGAGGACGCGAACGCTGTTGAAAAGGCAATCGCACTGCAGATACCCTTCTTTATGCTG
  GAGAATTCCGACCATGAAGTGCTTCGGACAGGCGAGAGTACGGAATTCACGTTCGATTTTGATTGCGAAT
  GGATCAACAACGAGCGCGCCTATATTCCCCCTGGCCTGATGTTCAACCCAAAGGTTCCCACTAGGAGAGC
  GCAATACATCAGGCAGCACGGGAACACGGCCTCCTCAAACACAAGAATTCAACCATATGCGAAGCCAACT
  TCCTGGATGACTGGACCTGGCCTGCTGAGTGCCCAGAGGGTTGGACCAGCCGGCTCTGATACCGCCAGCT
  GGATGGTGGTGGTCAACCCAGACGGAGCCGCTGTTAATTCCGGGATGGCCGGCGTTGGGTCCGGTTTCGA
  CCCCCCAAGCGGATCCCTGCGCCCCACGGATCTGGAGTATAAGATTCAGTGGTATCAGACCCCAGCCGGG
  ACAAATAGCGACGGCAATATTATCAGCAACCCGCCTCTGTCTATGCTTCGGGACCAGGCCCTGTACCGGG
  GAAATCAGACCACCTACAACCTGTGTAGCGACGTATGGATGTTTCCCAATCAGATTTGGGACAGATACCC
  CATAACACGGGAGAACCCAATCTGGTGTAAGAAACCTCGCAGTGATAAAAACACCATCATAGACCCGTTC
  GACGGGACACTGGCCATGGATCACCCGCCGGGCACCATTTTTATTAAGATGGCCAAGATTCCGGTCCCTT
  CTAACAACAATGCTGATAGTTACCTGAACATCTACTGTACCGGCCAAGTTAGTTGTGAGATCGTGTGGGA
  AGTGGAACGGTACGCTACTAAAAACTGGCGCCCTGAACGGAGGCATACCGCACTCGGTTTGGGGATCGGC
  GGAGAGGAGAACGTTAATCCTACCTACCATGTGGACAAGAATGGAAAATACATTCAGCCCACTACTTGGG
  ACATGTGCTACCCCATTAAGACTAATATTAATAAAGTGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100546 representing YP_002586775
  Red=Cloning sites Green=Tags
  
  MPPIKRQPGGWVLPGYKYLGPFNPLENGEPVNKADRAAQAHDKSYSEIIKSGKNPYLYFNKADEKFIDDL
  KNDWSLGGIIGSSFFKLKRAVAPALGNKERAQKRHFYFANSNKGAKKPKNNEPKPGTSKMSENEIQDQQP
  SGSMEERGGGGGAVGSVGGGKGSSVGISTGGWVGGSYFTDSYVITKNTRQFLVKIQNDHKYRTENIIPSN
  AGGKSQRCVSTPWSYFNFNQYSSHFSPQDWQHLTNEYKRFKPRKMHVKIYNLQIKQILSNGADTTYNNDL
  TAGVHIFCDGEHAYPNATHPWDEDVMPELPYETWYLFQYGYIPVIHELAEMEDANAVEKAIALQIPFFML
  ENSDHEVLRTGESTEFTFDFDCEWINNERAYIPPGLMFNPKVPTRRAQYIRQHGNTASSNTRIQPYAKPT
  SWMTGPGLLSAQRVGPAGSDTASWMVVVNPDGAAVNSGMAGVGSGFDPPSGSLRPTDLEYKIQWYQTPAG
  TNSDGNIISNPPLSMLRDQALYRGNQTTYNLCSDVWMFPNQIWDRYPITRENPIWCKKPRSDKNTIIDPF
  DGTLAMDHPPGTIFIKMAKIPVPSNNNADSYLNIYCTGQVSCEIVWEVERYATKNWRPERRHTALGLGIG
  GEENVNPTYHVDKNGKYIQPTTWDMCYPIKTNINKVL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012042
• ORF Size: 2001 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012042.1, YP_002586775
• RefSeq ORF: 2001
• Locus ID: 7755624
• MW: 74.9 kDa