VP1 (NC_012564) Virus Tagged ORF Clone

CAT#: VC100550

Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human bocavirus 3], codon optimized for human cell expression, YP_002808456

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100550 represents NCBI reference of YP_002808456 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCCCATTAAGCGCCAGCCAGGGGGATGGGTCCTCCCCGGCTATAAGTATCTTGGGCCTTTTAACC
  CACTGGATAATGGCGAGCCTGTGAATAAAGCAGATCGAGCGGCTCAAAGTCACGATAAGTCCTATAGCGA
  GCTGATCAAGTCAGGTAAGAACCCTTATCTGTACTTCAACAAAGCAGACGAGAAATTCATCGACGACCTC
  AAAAATGACTGGTCCTTGGGAGGCATCATAGGATCCAGCTTCTTTAAGTTGAAGAGGGCTGTGGCTCCCG
  CTCTGGGCAACAAAGAGAGAGCCCAGAAACGACACTTTTATTTTGCAAATTCAAATAAGGGCGCAAAGAA
  ATCCAAGAACAACGAACCTAAGCCAAGTACCTCTAAGATGAGCGAAAACGAGATTCAGGATCAGCAGCCC
  TCCGAGCCCAATGATGGTCAGCGCGGGGGGGGAGGTGGAGCTACCGGCTCTGTCGGTGGGGGGAAGGGCA
  GTGGAGTGGGAATCTCTACAGGAGGATGGGTGGGGGGGAGCTACTTTACAGACTCTTATGTGATCACTAA
  AAACACGCGGCAGTTTCTGGTTAAAATCCAAAATAATCACCAGTACAAGACCGAAAGCATTATTCCTAGC
  AACGGAGGAGGAAAAAGCCAACGATGTGTTAGCACGCCCTGGTCTTACTTTAATTTCAATCAATATTCTT
  CTCACTTCAGCCCACAGGACTGGCAACGGCTCACAAACGAATACAAGCGGTTTAGACCGAAGGGAATGCA
  CGTCAAAATTTACAATTTGCAGATCAAACAGATTCTTAGCAATGGCGCAGACGTGACCTACAACAATGAT
  CTCACAGCCGGAGTGCACATTTTCTGCGATGGCGAGCACGCTTATCCCAACGCCACACACCCATGGGATG
  AAGATGTTATGCCCGAGTTGCCTTACCAAACCTGGTACCTTTTCCAGTACGGCTACATCCCTACCATTCA
  TGAACTGGCTGAGATGGAGGATAGTAATGCTGTTGAGAAAGCAATCGCCCTGCAGATACCTTTCTTTATG
  CTCGAGAACTCCGACCACGAGGTTCTCAGGACAGGCGAGTCAGCCGAATTCAATTTCAATTTCGACTGTG
  AGTGGATAAATAACGAGCGGGCTTTCATACCACCCGGACTCATGTTTAACCCTCTCGTGCCGACCCGAAG
  GGCACAATACATTCGCAGAAATGGAAACACTCAAGCCAGTACATCTAGGGTGCAACCTTACGCGAAACCC
  ACTTCCTGGATGACAGGCCCTGGACTGTTGAGCGCACAAAGGGTCGGACCCGCCGCTTCTGATACAGCCG
  CCTGGATGGTGGGCGTCGATCCTGAAGGGGCCAACATCAACTCAGGTAGAGCTGGCGTTAGTTCTGGATT
  CGACCCTCCAGCCGGATCCCTTAGGCCAACAGATCTCGAGTACAAAGTGCAGTGGTACCAGACGCCCGCC
  GGCACAAATAATGATGGGAACATAATTTCCAATCCCCCGCTGTCTATGCTCCGGGACCAGACACTGTATC
  GGGGCAACCAGACCACGTATAACCTGTGTAGCGACGTTTGGATGTTCCCAAATCAGATCTGGGACAGATA
  TCCAGTTACGCGGGAGAACCCAATTTGGTGTAAACAGCCCCGGTCTGACAAGCATACCACTATCGATCCA
  TTTGACGGTTCCATAGCCATGGATCATCCTCCTGGTACTATCTTTATAAAAATGGCCAAGATCCCAGTGC
  CTTCAAACAATAACGCAGATAGCTACCTGAACATATATTGCACCGGTCAGGTTTCATGTGAGATCGTGTG
  GGAGGTGGAAAGGTACGCAACTAAGAATTGGCGCCCAGAACGCCGACACACAGCCCTTGGTCTGGGAATA
  GGTGGCGCCGATGAAATTAATCCCACCTACCATGTCGATAAGAACGGAGCCTATATTCAGCCAACTACCT
  GGGATATGTGCTTCCCAGTGAAAACCAATATCAATAAGGTATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100550 representing YP_002808456
  Red=Cloning sites Green=Tags
  
  MPPIKRQPGGWVLPGYKYLGPFNPLDNGEPVNKADRAAQSHDKSYSELIKSGKNPYLYFNKADEKFIDDL
  KNDWSLGGIIGSSFFKLKRAVAPALGNKERAQKRHFYFANSNKGAKKSKNNEPKPSTSKMSENEIQDQQP
  SEPNDGQRGGGGGATGSVGGGKGSGVGISTGGWVGGSYFTDSYVITKNTRQFLVKIQNNHQYKTESIIPS
  NGGGKSQRCVSTPWSYFNFNQYSSHFSPQDWQRLTNEYKRFRPKGMHVKIYNLQIKQILSNGADVTYNND
  LTAGVHIFCDGEHAYPNATHPWDEDVMPELPYQTWYLFQYGYIPTIHELAEMEDSNAVEKAIALQIPFFM
  LENSDHEVLRTGESAEFNFNFDCEWINNERAFIPPGLMFNPLVPTRRAQYIRRNGNTQASTSRVQPYAKP
  TSWMTGPGLLSAQRVGPAASDTAAWMVGVDPEGANINSGRAGVSSGFDPPAGSLRPTDLEYKVQWYQTPA
  GTNNDGNIISNPPLSMLRDQTLYRGNQTTYNLCSDVWMFPNQIWDRYPVTRENPIWCKQPRSDKHTTIDP
  FDGSIAMDHPPGTIFIKMAKIPVPSNNNADSYLNIYCTGQVSCEIVWEVERYATKNWRPERRHTALGLGI
  GGADEINPTYHVDKNGAYIQPTTWDMCFPVKTNINKVL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012564
• ORF Size: 2004 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012564.1, YP_002808456
• RefSeq ORF: 2004
• Locus ID: 7768238
• MW: 74.8 kDa