VP2 (NC_012564) Virus Tagged ORF Clone

CAT#: VC100551

Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human bocavirus 3], codon optimized for human cell expression, YP_002808457

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100551 represents NCBI reference of YP_002808457 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGAGAACGAAATCCAGGATCAGCAGCCCAGTGAGCCAAACGATGGACAGAGAGGGGGAGGCGGGG
  GAGCAACTGGGTCAGTGGGGGGCGGTAAAGGGAGTGGAGTTGGAATATCTACCGGCGGTTGGGTTGGTGG
  GAGCTATTTCACTGATTCATATGTCATAACAAAGAATACCCGACAGTTTCTGGTGAAGATCCAGAACAAC
  CACCAGTATAAGACAGAATCCATCATCCCTTCCAATGGGGGCGGCAAATCTCAAAGGTGCGTGTCCACCC
  CCTGGTCCTACTTTAACTTCAATCAATACTCAAGTCACTTCTCCCCTCAAGACTGGCAGCGCCTGACCAA
  CGAATACAAGCGCTTCCGCCCCAAGGGAATGCACGTGAAGATTTACAATTTGCAAATTAAACAAATCTTG
  TCTAACGGCGCTGACGTGACATACAACAATGACCTGACCGCAGGCGTGCACATTTTTTGCGACGGAGAGC
  ACGCTTATCCAAATGCAACCCATCCATGGGATGAAGACGTGATGCCGGAGCTCCCTTACCAGACTTGGTA
  CCTGTTTCAGTATGGGTATATCCCCACAATCCATGAACTGGCAGAGATGGAGGATTCTAATGCAGTAGAG
  AAAGCCATCGCCCTCCAGATTCCCTTTTTCATGCTGGAGAATTCAGATCACGAAGTCCTTCGCACCGGTG
  AGAGTGCGGAGTTTAATTTTAACTTCGATTGCGAGTGGATTAACAATGAAAGAGCATTCATCCCCCCTGG
  ACTGATGTTTAATCCCCTGGTACCTACTCGGCGGGCGCAATACATCAGAAGAAATGGCAACACTCAGGCT
  TCAACATCCCGGGTACAGCCGTACGCAAAACCCACAAGCTGGATGACTGGGCCTGGCCTCCTCTCCGCAC
  AGAGGGTGGGTCCTGCCGCCTCCGATACCGCTGCATGGATGGTAGGAGTAGACCCTGAGGGCGCTAATAT
  CAACTCAGGAAGGGCTGGCGTTAGCAGTGGCTTTGATCCTCCGGCTGGGAGCCTTAGGCCCACAGACCTT
  GAGTACAAAGTTCAGTGGTATCAAACCCCAGCCGGGACAAACAACGACGGAAATATTATATCCAACCCCC
  CCTTGTCAATGCTCCGAGATCAGACACTCTACCGGGGTAATCAGACTACATACAATCTGTGCAGCGATGT
  GTGGATGTTCCCCAATCAGATCTGGGACAGATACCCTGTAACCCGAGAGAACCCTATATGGTGCAAGCAG
  CCCAGGTCAGATAAGCACACAACAATTGATCCGTTTGATGGTTCCATTGCTATGGATCACCCCCCCGGAA
  CCATATTTATCAAGATGGCTAAGATACCTGTGCCCTCCAATAATAATGCTGACTCTTATCTGAATATCTA
  TTGTACAGGCCAAGTCAGCTGTGAGATCGTCTGGGAGGTCGAGAGGTACGCCACCAAGAATTGGCGACCC
  GAAAGGCGGCATACTGCCCTCGGACTCGGAATCGGAGGGGCCGATGAGATCAACCCAACCTACCATGTGG
  ATAAGAATGGCGCCTACATCCAGCCCACCACATGGGATATGTGTTTCCCAGTGAAGACAAATATAAACAA
  GGTGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100551 representing YP_002808457
  Red=Cloning sites Green=Tags
  
  MSENEIQDQQPSEPNDGQRGGGGGATGSVGGGKGSGVGISTGGWVGGSYFTDSYVITKNTRQFLVKIQNN
  HQYKTESIIPSNGGGKSQRCVSTPWSYFNFNQYSSHFSPQDWQRLTNEYKRFRPKGMHVKIYNLQIKQIL
  SNGADVTYNNDLTAGVHIFCDGEHAYPNATHPWDEDVMPELPYQTWYLFQYGYIPTIHELAEMEDSNAVE
  KAIALQIPFFMLENSDHEVLRTGESAEFNFNFDCEWINNERAFIPPGLMFNPLVPTRRAQYIRRNGNTQA
  STSRVQPYAKPTSWMTGPGLLSAQRVGPAASDTAAWMVGVDPEGANINSGRAGVSSGFDPPAGSLRPTDL
  EYKVQWYQTPAGTNNDGNIISNPPLSMLRDQTLYRGNQTTYNLCSDVWMFPNQIWDRYPVTRENPIWCKQ
  PRSDKHTTIDPFDGSIAMDHPPGTIFIKMAKIPVPSNNNADSYLNIYCTGQVSCEIVWEVERYATKNWRP
  ERRHTALGLGIGGADEINPTYHVDKNGAYIQPTTWDMCFPVKTNINKVL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012564
• ORF Size: 1617 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012564.1, YP_002808457
• RefSeq ORF: 1617
• Locus ID: 7768239
• MW: 60.4 kDa