spike protein (NC_005831) Virus Tagged ORF Clone

CAT#: VC100593

Myc-DDK-tagged ORF clone of viral ORF for spike protein [Human coronavirus NL63], codon optimized for human cell expression, YP_003767

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: spike protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100593 represents NCBI reference of YP_003767 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTCTTTTTGATCCTGCTGGTCCTGCCACTGGCCAGCTGTTTCTTTACCTGCAATTCAAATGCCA
  ATCTGTCAATGCTGCAACTCGGAGTCCCCGATAATAGCAGTACTATCGTCACAGGGCTTTTGCCGACCCA
  TTGGTTCTGTGCTAACCAGTCTACCTCCGTCTATAGCGCCAACGGATTCTTCTACATTGATGTGGGAAAC
  CACAGGAGCGCCTTCGCTTTGCATACCGGGTACTATGATGCTAACCAGTACTACATTTACGTCACCAACG
  AAATCGGTTTGAATGCAAGCGTGACGTTGAAGATCTGTAAGTTTTCACGAAATACAACGTTTGATTTCCT
  GTCCAATGCTAGCAGCAGTTTTGATTGCATTGTGAACCTTTTGTTCACTGAGCAGCTCGGGGCTCCTCTT
  GGTATTACCATCTCAGGGGAAACTGTCCGACTGCACTTGTACAACGTGACTAGAACATTTTACGTCCCTG
  CAGCGTACAAGCTCACCAAGCTCAGTGTGAAATGCTATTTTAATTACTCCTGCGTATTCAGCGTTGTGAA
  CGCGACCGTGACCGTAAACGTCACAACCCATAATGGGAGGGTAGTGAACTATACAGTATGTGATGACTGC
  AACGGTTATACCGATAACATTTTTTCTGTACAGCAGGATGGACGAATTCCAAATGGGTTTCCATTTAACA
  ACTGGTTCCTCCTGACAAACGGATCCACTCTGGTGGACGGAGTTTCTAGGCTGTACCAGCCTTTGAGATT
  GACCTGTCTCTGGCCAGTGCCCGGTCTCAAATCCTCTACTGGCTTTGTGTATTTTAACGCGACTGGATCC
  GATGTGAACTGTAACGGTTACCAGCACAATTCCGTTGTGGATGTGATGCGGTACAACCTGAACTTCAGCG
  CCAATAGCCTTGATAATTTGAAAAGCGGTGTCATTGTTTTCAAAACTCTTCAGTACGACGTCCTTTTCTA
  CTGCAGTAATAGTAGTAGCGGTGTTCTCGACACCACTATTCCTTTCGGTCCCTCTTCTCAGCCCTACTAC
  TGCTTCATTAATTCAACGATTAACACTACTCATGTGAGTACCTTTGTGGGTATTTTGCCCCCCACCGTTC
  GCGAGATCGTCGTAGCTAGGACTGGGCAGTTCTACATCAACGGATTCAAGTATTTCGATCTCGGCTTTAT
  AGAGGCAGTGAACTTCAACGTTACCACGGCCTCAGCAACAGATTTCTGGACGGTCGCATTTGCAACGTTC
  GTGGACGTGCTTGTCAACGTGTCTGCGACCAATATTCAGAATCTTCTGTACTGTGATAGCCCCTTTGAAA
  AATTGCAGTGCGAGCATCTTCAATTCGGATTGCAGGATGGCTTTTATTCTGCGAATTTTTTGGACGACAA
  TGTGCTGCCTGAGACCTACGTCGCATTGCCCATCTATTACCAGCATACGGATATCAATTTTACGGCAACC
  GCATCCTTTGGGGGTTCCTGCTACGTTTGCAAGCCCCACCAGGTCAATATAAGCCTCAATGGCAATACCT
  CCGTCTGCGTGAGGACTTCTCACTTTTCAATAAGATACATTTACAACAGAGTGAAGAGTGGATCTCCCGG
  CGACTCCTCTTGGCACATCTACCTGAAATCCGGCACATGTCCCTTCTCTTTTAGCAAGCTGAATAATTTC
  CAGAAGTTCAAAACAATCTGCTTCAGCACCGTTGAGGTTCCAGGTTCTTGTAATTTCCCCCTGGAAGCCA
  CCTGGCACTACACATCATATACCATCGTCGGAGCGCTGTATGTGACTTGGAGCGAAGGCAACTCCATTAC
  GGGGGTTCCCTACCCAGTCAGCGGAATACGGGAATTCTCAAATCTGGTATTGAATAACTGCACAAAATAC
  AACATCTATGACTACGTGGGAACAGGGATCATCCGGTCCTCTAATCAGTCCCTGGCTGGCGGCATTACGT
  ACGTCAGCAACTCTGGAAATCTGCTTGGGTTCAAGAACGTATCAACAGGCAATATTTTTATCGTTACTCC
  GTGCAATCAGCCGGACCAGGTCGCTGTTTACCAGCAATCTATCATTGGTGCCATGACAGCAGTTAACGAA
  AGCCGCTACGGATTGCAGAACTTGCTGCAGCTTCCTAACTTTTACTACGTGTCTAATGGCGGAAATAATT
  GTACCACTGCAGTGATGACATATTCCAACTTCGGGATCTGTGCGGACGGATCTCTGATCCCAGTACGGCC
  AAGAAATTCAAGTGACAACGGAATTTCCGCGATTATTACCGCTAACCTGTCAATTCCCAGCAATTGGACG
  ACCTCTGTGCAGGTCGAGTACCTGCAAATCACATCCACTCCCATAGTCGTCGACTGCGCCACCTATGTGT
  GTAACGGTAATCCCAGGTGCAAAAATCTGTTGAAACAGTATACATCAGCCTGCAAAACAATCGAGGACGC
  CCTTCGCCTCTCCGCTCACTTGGAAACTAACGACGTATCTTCAATGCTGACATTCGACAGTAATGCCTTT
  TCCCTCGCAAACGTTACATCCTTCGGAGACTATAACCTCTCATCCGTGCTCCCCCAAAGGAATATCAGGT
  CCAGTAGGATCGCCGGACGGTCTGCACTGGAGGACCTGTTGTTCAGTAAAGTCGTGACTAGTGGCTTGGG
  CACTGTGGACGTGGACTACAAATCTTGTACTAAGGGATTGAGTATCGCGGACCTTGCATGTGCCCAATAT
  TACAATGGGATCATGGTACTGCCAGGGGTGGCTGACGCAGAGCGGATGGCAATGTACACAGGCAGCCTTA
  TCGGCGGGATGGTCTTGGGGGGCCTGACCAGTGCCGCGGCGATCCCATTCTCCCTGGCGCTGCAAGCCCG
  GCTCAACTACGTGGCCTTGCAGACTGACGTCCTTCAAGAGAACCAGAAAATTCTGGCAGCTAGCTTCAAC
  AAAGCCATTAATAATATCGTTGCCTCCTTCAGCTCAGTTAATGACGCCATTACACAGACGGCTGAGGCCA
  TCCATACAGTGACTATTGCGCTGAATAAGATCCAAGACGTAGTGAACCAGCAGGGGAGCGCCCTGAACCA
  CCTTACCTCCCAACTTCGCCATAACTTTCAGGCAATTTCAAATTCAATACAGGCAATTTATGACCGGCTC
  GACTCCATCCAGGCTGATCAGCAGGTGGACCGCCTGATAACCGGGCGCCTCGCCGCCCTCAACGCTTTCG
  TTAGTCAAGTGCTGAATAAGTACACAGAGGTGAGAGGCAGCCGGAGGTTGGCCCAGCAGAAGATCAATGA
  ATGCGTGAAGTCCCAATCCAATCGGTATGGTTTCTGCGGCAACGGCACACACATTTTCTCAATAGTGAAC
  TCTGCCCCAGATGGCCTCCTGTTTCTCCACACCGTACTGCTGCCTACTGATTATAAAAATGTGAAGGCCT
  GGAGTGGCATCTGCGTGGACGGTATATATGGCTATGTTTTGCGGCAGCCCAACTTGGTGCTGTACTCCGA
  CAACGGAGTGTTTCGCGTGACTTCCAGAGTTATGTTCCAGCCAAGATTGCCCGTCCTCTCTGATTTCGTA
  CAAATCTATAATTGTAACGTTACTTTTGTGAACATTAGTCGAGTGGAACTCCACACCGTCATTCCAGATT
  ACGTGGACGTCAATAAAACCCTCCAAGAGTTCGCTCAGAATCTCCCCAAGTATGTCAAGCCTAACTTCGA
  TCTCACGCCTTTTAACCTTACCTACCTGAACCTGAGTTCCGAACTGAAACAACTGGAAGCAAAGACCGCT
  AGCCTGTTTCAGACAACCGTCGAACTCCAGGGCTTGATAGACCAGATCAACAGCACCTATGTGGACCTGA
  AGCTGCTCAACCGATTTGAAAATTACATCAAATGGCCGTGGTGGGTGTGGCTGATCATCTCCGTCGTTTT
  CGTGGTCCTGCTTTCCCTTCTGGTGTTCTGTTGCCTTAGCACCGGATGCTGTGGTTGCTGCAACTGTCTT
  ACATCATCTATGAGGGGCTGCTGTGATTGCGGAAGCACTAAGCTCCCTTATTACGAGTTCGAAAAGGTAC
  ACGTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100593 representing YP_003767
  Red=Cloning sites Green=Tags
  
  MKLFLILLVLPLASCFFTCNSNANLSMLQLGVPDNSSTIVTGLLPTHWFCANQSTSVYSANGFFYIDVGN
  HRSAFALHTGYYDANQYYIYVTNEIGLNASVTLKICKFSRNTTFDFLSNASSSFDCIVNLLFTEQLGAPL
  GITISGETVRLHLYNVTRTFYVPAAYKLTKLSVKCYFNYSCVFSVVNATVTVNVTTHNGRVVNYTVCDDC
  NGYTDNIFSVQQDGRIPNGFPFNNWFLLTNGSTLVDGVSRLYQPLRLTCLWPVPGLKSSTGFVYFNATGS
  DVNCNGYQHNSVVDVMRYNLNFSANSLDNLKSGVIVFKTLQYDVLFYCSNSSSGVLDTTIPFGPSSQPYY
  CFINSTINTTHVSTFVGILPPTVREIVVARTGQFYINGFKYFDLGFIEAVNFNVTTASATDFWTVAFATF
  VDVLVNVSATNIQNLLYCDSPFEKLQCEHLQFGLQDGFYSANFLDDNVLPETYVALPIYYQHTDINFTAT
  ASFGGSCYVCKPHQVNISLNGNTSVCVRTSHFSIRYIYNRVKSGSPGDSSWHIYLKSGTCPFSFSKLNNF
  QKFKTICFSTVEVPGSCNFPLEATWHYTSYTIVGALYVTWSEGNSITGVPYPVSGIREFSNLVLNNCTKY
  NIYDYVGTGIIRSSNQSLAGGITYVSNSGNLLGFKNVSTGNIFIVTPCNQPDQVAVYQQSIIGAMTAVNE
  SRYGLQNLLQLPNFYYVSNGGNNCTTAVMTYSNFGICADGSLIPVRPRNSSDNGISAIITANLSIPSNWT
  TSVQVEYLQITSTPIVVDCATYVCNGNPRCKNLLKQYTSACKTIEDALRLSAHLETNDVSSMLTFDSNAF
  SLANVTSFGDYNLSSVLPQRNIRSSRIAGRSALEDLLFSKVVTSGLGTVDVDYKSCTKGLSIADLACAQY
  YNGIMVLPGVADAERMAMYTGSLIGGMVLGGLTSAAAIPFSLALQARLNYVALQTDVLQENQKILAASFN
  KAINNIVASFSSVNDAITQTAEAIHTVTIALNKIQDVVNQQGSALNHLTSQLRHNFQAISNSIQAIYDRL
  DSIQADQQVDRLITGRLAALNAFVSQVLNKYTEVRGSRRLAQQKINECVKSQSNRYGFCGNGTHIFSIVN
  SAPDGLLFLHTVLLPTDYKNVKAWSGICVDGIYGYVLRQPNLVLYSDNGVFRVTSRVMFQPRLPVLSDFV
  QIYNCNVTFVNISRVELHTVIPDYVDVNKTLQEFAQNLPKYVKPNFDLTPFNLTYLNLSSELKQLEAKTA
  SLFQTTVELQGLIDQINSTYVDLKLLNRFENYIKWPWWVWLIISVVFVVLLSLLVFCCLSTGCCGCCNCL
  TSSMRGCCDCGSTKLPYYEFEKVHVQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005831
• ORF Size: 4068 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005831.2, YP_003767
• RefSeq ORF: 4068
• Locus ID: 2943499
• MW: 149.9 kDa