protein 3 (NC_005831) Virus Tagged ORF Clone

CAT#: VC100594

Myc-DDK-tagged ORF clone of viral ORF for protein 3 [Human coronavirus NL63], codon optimized for human cell expression, YP_003768

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: protein 3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100594 represents NCBI reference of YP_003768 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTTTGGAGGTCTGTTTCAGCTCACCCTGGAAAGTACTATTAACAAAAGCGTGGCCAACCTCAAGC
  TCCCACCTCACGACGTGACAGTGCTGAGAGATAACCTGAAGCCTGTCACTACTCTGTCTACCATCACCGC
  CTACCTGTTGGTGTCCCTTTTCGTTACCTACTTTGCATTGTTCAAGCCTCTGACCGCAAGAGGCAGGGTT
  GCATGTTTCGTCCTGAAACTCCTCACATTGTTTGTGTATGTTCCACTGCTGGTGCTGTTCGGGATGTATC
  TGGACAGTTTCATCATTTTCTCCACCCTCCTGTTCCGCTTTATCCACGTGGGCTATTATGCCTACCTGTA
  CAAAAACTTTTCCTTCGTCTTGTTTAACGTGACGAAACTCTGTTTCGTGTCAGGCAAGTGTTGGTATCTT
  GAGCAGTCCTTCTACGAAAATAGGTTCGCTGCCATATATGGGGGGGATCACTACGTAGTGCTGGGCGGCG
  AGACCATCACCTTTGTCAGCTTTGACGACCTGTACGTGGCCATTCGCGGCTCATGCGAGAAGAATCTTCA
  ACTGATGCGAAAAGTGGACCTGTATAACGGAGCCGTAATTTACATTTTCGCCGAAGAACCCGTCGTGGGA
  ATTGTCTACTCTTCACAACTGTACGAGGATGTACCCAGTATTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100594 representing YP_003768
  Red=Cloning sites Green=Tags
  
  MPFGGLFQLTLESTINKSVANLKLPPHDVTVLRDNLKPVTTLSTITAYLLVSLFVTYFALFKPLTARGRV
  ACFVLKLLTLFVYVPLLVLFGMYLDSFIIFSTLLFRFIHVGYYAYLYKNFSFVLFNVTKLCFVSGKCWYL
  EQSFYENRFAAIYGGDHYVVLGGETITFVSFDDLYVAIRGSCEKNLQLMRKVDLYNGAVIYIFAEEPVVG
  IVYSSQLYEDVPSIN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005831
• ORF Size: 675 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005831.2, YP_003768
• RefSeq ORF: 675
• Locus ID: 2943500
• MW: 25.7 kDa