membrane protein (NC_005831) Virus Tagged ORF Clone

CAT#: VC100596

Myc-DDK-tagged ORF clone of viral ORF for membrane protein [Human coronavirus NL63], codon optimized for human cell expression, YP_003770

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: membrane protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100596 represents NCBI reference of YP_003770 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAACAGTAGCGTCCCCTTGCTGGAGGTGTACGTACACCTTAGAAACTGGAACTTCAGCTGGAATC
  TGATCTTGACCCTGTTCATCGTAGTGCTGCAGTACGGGCACTATAAGTACTCACGCCTGTTGTATGGCCT
  TAAGATGTCCGTCCTTTGGTGTCTGTGGCCCCTTGTACTGGCACTTTCAATTTTCGATTGTTTCGTGAAC
  TTCAATGTGGACTGGGTTTTTTTCGGATTTAGCATTTTGATGTCCATCATTACATTGTGCTTGTGGGTGA
  TGTACTTCGTAAACTCATTTCGATTGTGGCGCCGAGTCAAAACCTTCTGGGCCTTCAACCCAGAAACAAA
  TGCCATTATTAGCTTGCAGGTGTACGGCCATAACTATTATCTCCCTGTTATGGCCGCCCCTACTGGTGTG
  ACTCTGACTCTGCTTAGCGGCGTCCTGCTTGTGGATGGCCACAAGATCGCGACAAGGGTTCAGGTGGGGC
  AGCTCCCTAAATACGTAATTGTGGCTACCCCATCCACTACAATCGTCTGCGACAGGGTCGGAAGATCTGT
  GAACGAGACCTCTCAAACCGGATGGGCCTTCTACGTGAGGGCGAAGCATGGCGATTTTAGCGGAGTAGCC
  TCACAGGAAGGTGTGCTGAGTGAGCGGGAAAAACTTCTCCACTTGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100596 representing YP_003770
  Red=Cloning sites Green=Tags
  
  MSNSSVPLLEVYVHLRNWNFSWNLILTLFIVVLQYGHYKYSRLLYGLKMSVLWCLWPLVLALSIFDCFVN
  FNVDWVFFGFSILMSIITLCLWVMYFVNSFRLWRRVKTFWAFNPETNAIISLQVYGHNYYLPVMAAPTGV
  TLTLLSGVLLVDGHKIATRVQVGQLPKYVIVATPSTTIVCDRVGRSVNETSQTGWAFYVRAKHGDFSGVA
  SQEGVLSEREKLLHLI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005831
• ORF Size: 678 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005831.2, YP_003770
• RefSeq ORF: 678
• Locus ID: 2943503
• MW: 25.9 kDa