E7 (NC_001356) Virus Tagged ORF Clone

CAT#: VC101764

Myc-DDK-tagged ORF clone of viral ORF for E7 protein [Mupapillomavirus 1], codon optimized for human cell expression, NP_040307

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101764 represents NCBI reference of NP_040307 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGCGAAATGCCTGCGCTTAAAGATCTCGTTCTGCAGTTGGAGCCCTCTGTTCTCGACCTCGACT
  TGTACTGCTATGAAGAAGTACCACCAGATGATATAGAGGAAGAGCTCGTCTCTCCACAACAGCCATACGC
  GGTGGTCGCCAGTTGCGCTTATTGTGAAAAACTTGTGAGGCTGACAGTGTTGGCAGACCACTCTGCAATT
  AGGCAGCTTGAAGAGCTGCTCCTGAGATCACTGAATATTGTTTGTCCTCTTTGCACACTCCAAAGACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101764 representing NP_040307
  Red=Cloning sites Green=Tags
  
  MVGEMPALKDLVLQLEPSVLDLDLYCYEEVPPDDIEEELVSPQQPYAVVASCAYCEKLVRLTVLADHSAI
  RQLEELLLRSLNIVCPLCTLQRQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001356
• ORF Size: 279 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001356.1, NP_040307
• RefSeq ORF: 279
• Locus ID: 1489171
• MW: 10.5 kDa