L1 (NC_001356) Virus Tagged ORF Clone

CAT#: VC101766

Myc-DDK-tagged ORF clone of viral ORF for major capsid protein L1 [Mupapillomavirus 1], codon optimized for human cell expression, NP_040309

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101766 represents NCBI reference of NP_040309 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATAACGTGTTTCAGATGGCGGTGTGGCTTCCTGCTCAGAACAAGTTCTACCTTCCACCCCAGCCAA
  TTACACGCATCCTGAGCACCGACGAGTACGTGACTCGGACGAACCTGTTCTACCATGCAACATCCGAGCG
  ACTGTTGCTGGTTGGGCATCCACTGTTTGAGATTTCCTCCAATCAGACAGTGACGATACCCAAAGTCAGT
  CCTAATGCCTTTCGGGTATTTCGGGTACGCTTCGCAGACCCAAACCGATTCGCTTTTGGCGACAAGGCCA
  TATTTAACCCCGAGACTGAAAGACTCGTGTGGGGTTTGAGAGGCATTGAAATCGGGAGGGGCCAGCCCCT
  TGGAATTGGCATCACCGGGCATCCTCTCCTGAACAAGCTTGACGATGCTGAGAATCCTACTAACTACATC
  AATACACATGCCAATGGCGACTCACGCCAGAATACAGCCTTCGACGCAAAGCAAACTCAGATGTTCCTCG
  TTGGGTGCACACCCGCCAGCGGCGAACATTGGACTAGCTCCCGGTGCCCAGGCGAGCAGGTGAAACTGGG
  AGACTGCCCTCGGGTCCAGATGATCGAATCCGTGATCGAAGATGGCGACATGATGGACATCGGCTTTGGG
  GCTATGGACTTTGCCGCACTCCAACAGGATAAATCAGATGTGCCACTGGACGTGGTGCAGGCCACCTGTA
  AGTACCCTGACTACATCCGCATGAACCACGAGGCTTACGGGAATTCCATGTTTTTCTTTGCTCGGCGGGA
  ACAGATGTACACACGACACTTTTTTACACGCGGCGGCTCAGTAGGGGATAAGGAGGCCGTGCCCCAGTCT
  CTCTACCTCACAGCTGATGCCGAGCCCCGGACTACGCTCGCCACCACCAACTATGTGGGCACCCCCTCTG
  GCTCTATGGTGTCTTCCGACGTTCAGCTGTTTAACAGGTCATATTGGCTCCAGCGCTGTCAAGGGCAAAA
  CAATGGAATCTGCTGGAGAAACCAACTGTTCATTACCGTTGGCGACAATACCAGAGGGACTAGTCTTTCT
  ATTTCAATGAAGAATAATGCGTCCACCACCTATTCCAACGCTAACTTTAACGACTTTCTGCGCCACACCG
  AAGAATTCGATCTGAGTTTTATTGTGCAGCTGTGTAAAGTTAAACTCACCCCCGAGAATCTGGCATATAT
  TCACACTATGGACCCTAACATCCTGGAGGACTGGCAGCTGTCCGTTAGCCAACCACCAACAAACCCCTTG
  GAAGATCAGTATCGCTTCCTGGGCTCATCTCTGGCCGCAAAGTGCCCTGAGCAGGCCCCACCCGAGCCAC
  AGACAGACCCTTACAGCCAATACAAGTTCTGGGAAGTCGACCTGACCGAGAGGATGTCAGAGCAGCTGGA
  CCAGTTTCCTCTGGGCAGGAAGTTTCTGTATCAGTCAGGTATGACTCAGCGCACTGCCACTAGCTCTACT
  ACCAAACGCAAGACTGTACGGGTCAGTACGAGTGCTAAACGGCGGAGAAAGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101766 representing NP_040309
  Red=Cloning sites Green=Tags
  
  MYNVFQMAVWLPAQNKFYLPPQPITRILSTDEYVTRTNLFYHATSERLLLVGHPLFEISSNQTVTIPKVS
  PNAFRVFRVRFADPNRFAFGDKAIFNPETERLVWGLRGIEIGRGQPLGIGITGHPLLNKLDDAENPTNYI
  NTHANGDSRQNTAFDAKQTQMFLVGCTPASGEHWTSSRCPGEQVKLGDCPRVQMIESVIEDGDMMDIGFG
  AMDFAALQQDKSDVPLDVVQATCKYPDYIRMNHEAYGNSMFFFARREQMYTRHFFTRGGSVGDKEAVPQS
  LYLTADAEPRTTLATTNYVGTPSGSMVSSDVQLFNRSYWLQRCQGQNNGICWRNQLFITVGDNTRGTSLS
  ISMKNNASTTYSNANFNDFLRHTEEFDLSFIVQLCKVKLTPENLAYIHTMDPNILEDWQLSVSQPPTNPL
  EDQYRFLGSSLAAKCPEQAPPEPQTDPYSQYKFWEVDLTERMSEQLDQFPLGRKFLYQSGMTQRTATSST
  TKRKTVRVSTSAKRRRKA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001356
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001356.1, NP_040309
• RefSeq ORF: 1524
• Locus ID: 1489173
• MW: 57.6 kDa