E1 (NC_001356) Virus Tagged ORF Clone

CAT#: VC101767

Myc-DDK-tagged ORF clone of viral ORF for replication protein E1 [Mupapillomavirus 1], codon optimized for human cell expression, NP_597739

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101767 represents NCBI reference of NP_597739 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACAACAAGGGAACAGAGAACGATTGGTTCCTTGTTGAAGCCACAGACTGTGAGGAGACCCTGG
  AGGAAACCTCCTTGGGAGATCTGGACAACGTATCCTGCGTGAGCGATCTTTCAGACCTCCTGGATGAGGC
  ACCGCAGAGCCAGGGCAACTCTCTCGAGCTTTTCCACAAGCAGGAGTCACTGGAGTCTGAGCAAGAGCTC
  AATGCCCTGAAAAGGAAATTGCTTTATTCACCCCAGGCAAGGAGTGCTGATGAAACGGATATTGCTAGTA
  TTTCTCCAAGACTCGAAACCATTTCCATCACGAAACAGGACAAGAAGCGGTATCGACGCCAGCTGTTCTC
  TCAGGATGACTCCGGCCTCGAGCTGTCCCTTCTTCAGGATGAAACAGAAAACATCGATGAGTCTACCCAA
  GTGGATCAGCAGCAGAAGGAACACACCGGTGAGGTGGGAGCCGCAGGTGTCAATATCCTGAAAGCGAGCA
  ACATCAGAGCAGCTCTCCTTTCCCGGTTTAAAGATACGGCTGGTGTGTCCTTCACAGATCTGACGAGAAG
  CTACAAAAGCAACAAGACCTGTTGTGGAGATTGGGTTCTGGCAGTATGGGGTGTCCGGGAGAATCTGATT
  GACAGTGTCAAAGAACTCCTGCAGACACATTGCGTCTATATCCAACTGGAGCATGCCGTTACTGAGAAAA
  ACAGATTCCTGTTCCTGCTTGTGCGGTTCAAGGCCCAAAAGTCCCGGGAGACGGTTATCAAGCTCATTAC
  TACCATCCTCCCAGTAGATGCCTCTTATATCCTCTCAGAACCACCAAAATCCAGGAGCGTGGCCGCCGCC
  CTTTTCTGGTATAAGCGCTCTATGAGCTCAACCGTTTTCACTTGGGGTACAACACTTGAGTGGATTGCAC
  AACAGACATTGATAAATCACCAATTGGACTCAGAGAGTCCGTTTGAACTCTGTAAGATGGTGCAATGGGC
  CTACGATAACGGTCATACGGAGGAGTGCAAGATTGCCTACTATTACGCAGTCCTGGCTGACGAAGATGAA
  AACGCCCGCGCATTCCTGAGCAGCAACTCTCAGGCGAAATATGTGAAGGATTGTGCCCAGATGGTCCGAC
  ACTACCTTAGGGCTGAGATGGCACAAATGTCAATGTCCGAGTGGATATTCCGGAAATTGGATAATGTGGA
  GGGCAGCGGGAACTGGAAGGAGATCGTTAGATTCTTGCGCTTCCAAGAAGTGGAGTTCATTAGCTTCATG
  ATCGCATTCAAAGACCTGCTGTGCGGCAAGCCTAAAAAGAACTGTCTCCTTATTTTCGGCCCTCCTAACA
  CGGGAAAGTCTATGTTCTGTACGTCTCTGTTGAAGTTGCTGGGCGGTAAAGTGATCTCCTACTGCAACTC
  CAAGAGCCAATTTTGGCTGCAGCCACTTGCAGATGCAAAGATCGGTCTGTTGGACGACGCAACCAAGCCA
  TGCTGGGACTATATGGATATATACATGCGGAACGCCCTTGACGGAAATACCATATGTATCGATCTCAAGC
  ATAGAGCACCCCAGCAGATCAAGTGTCCACCTCTGCTGATCACCTCCAATATTGACGTCAAATCCGATAC
  GTGCTGGATGTATCTCCATAGCAGGATTAGCGCTTTTAAGTTCGCACATGAGTTCCCATTTAAAGACAAC
  GGCGATCCAGGGTTTAGTCTGACAGATGAAAATTGGAAGAGCTTTTTTGAGAGATTCTGGCAGCAACTGG
  AGCTGTCCGACCAGGAAGACGAGGGCAACGATGGTAAGCCCCAGCAGTCACTGAGACTTACAGCCCGGGC
  TGCAAATGAGCCGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101767 representing NP_597739
  Red=Cloning sites Green=Tags
  
  MADNKGTENDWFLVEATDCEETLEETSLGDLDNVSCVSDLSDLLDEAPQSQGNSLELFHKQESLESEQEL
  NALKRKLLYSPQARSADETDIASISPRLETISITKQDKKRYRRQLFSQDDSGLELSLLQDETENIDESTQ
  VDQQQKEHTGEVGAAGVNILKASNIRAALLSRFKDTAGVSFTDLTRSYKSNKTCCGDWVLAVWGVRENLI
  DSVKELLQTHCVYIQLEHAVTEKNRFLFLLVRFKAQKSRETVIKLITTILPVDASYILSEPPKSRSVAAA
  LFWYKRSMSSTVFTWGTTLEWIAQQTLINHQLDSESPFELCKMVQWAYDNGHTEECKIAYYYAVLADEDE
  NARAFLSSNSQAKYVKDCAQMVRHYLRAEMAQMSMSEWIFRKLDNVEGSGNWKEIVRFLRFQEVEFISFM
  IAFKDLLCGKPKKNCLLIFGPPNTGKSMFCTSLLKLLGGKVISYCNSKSQFWLQPLADAKIGLLDDATKP
  CWDYMDIYMRNALDGNTICIDLKHRAPQQIKCPPLLITSNIDVKSDTCWMYLHSRISAFKFAHEFPFKDN
  GDPGFSLTDENWKSFFERFWQQLELSDQEDEGNDGKPQQSLRLTARAANEPI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001356
• ORF Size: 1836 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001356.1, NP_597739
• RefSeq ORF: 1836
• Locus ID: 1724533
• MW: 69.9 kDa