E1 (NC_001352) Virus Tagged ORF Clone

CAT#: VC101813

Myc-DDK-tagged ORF clone of viral ORF for early protein E1 [Alphapapillomavirus 4], codon optimized for human cell expression, NP_077118

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101813 represents NCBI reference of NP_077118 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACTCAGAGGGGACCGACGGTACCGAGGAGGATGGTTGCCGGGCGGGGGGCTGGTTTCACGTTG
  AAGCTATCATTACCCACGGTCAGCGGCAGGTATCATCAGATGAAGACGAGGACGAGACCGAGACCGGCGA
  GGACCTCGACTTCATCGATAATCGCGTTCCCGGTGATGGACAAGAGATTCCTCTGCAGCTGTACGCACAA
  CAAACTGCCCAGGATGATGAAGCCACAGTGCAGGCCTTGAAGCGCAAGTTCGTCGCTTCCCCTTTGTCCG
  CCTGCTCTTGCATCGAAAACGACCTGTCCCCCCGGCTGGACGCCATCAGTCTCAATCGGAAATCTGAGAA
  GGCTAAGCGCAGGCTTTTCGAGACCGAGCCTCCCGACAGTGGATATGGCAATACACAAATGGTGGTGGGG
  ACACCGGAAGAGGTCACCGGCGACGAGGAATCTCAGGGTGGGAGACCTGTGGAAGACCAGGAAGAGGAGC
  GCCAAGGCGGCGATGGCGAGGCTGATCTCACAGTGCATACACCGCAGTCTGGCACAGATGCAGCTGGGTC
  TGTCCTGACCTTGTTGCGAAGTAGCAACCTGAAAGCGACACTTCTGAGCAAATTTAAAGACCTGTTTGGC
  GTAGGCTTTTACGAACTCGTGCGGCAGTTCAAAAGCTCTAAGACCGCCTGCGCCGATTGGGTAGTGTGCG
  CCTACGGGGTATATTACGCGGTCGCTGAAGGACTCAAGAAACTGATCCAACCACACACCCAGTACGCCCA
  TATCCAGGTGCAGACCTCTTCTTGGGGCATGGTCGTTTTCATGCTCCTCCGCTATAACTGCGCAAAGAAC
  CGAGACAGTGTCTCCAAAAATATGAGCATGCTTTTGAACATTCCTGAGAAGCATATGTTGATAGAGCCCC
  CTAAGCTGCGCAGCACACCGGCTGCGCTTTACTGGTACAAAACAGCCATGGGAAATGGTTCCGAGGTGTA
  TGGCGAAACACCTGAATGGATCGTGCGGCAAACCCTTGTGGGACATTCAATGGAGGATGAACAGTTCAGG
  CTGAGTGTGATGGTGCAATATGCATACGACCACGACATTGTCGAGGAAAGCGTGCTCGCATTCGAGTACG
  CACAGCTGGCCGATGTAGATGCCAATGCGGCTGCATTCCTGAATTCTAACTGCCAGGCTAAGTATGTGAA
  AGATGCTGTCACCATGTGCCGACATTACAAGAGGGCCGAGAGGGAGCAGATGTCTATGTCACAGTGGATT
  ACCTTTAGGGGAAATAAGGTGTCTGAAGAGGGGGATTGGAAGCCAATTGTTAGATTCCTGCGGCACCAAG
  GCGTGGAGTTCGTCAGCTTCCTGGCTGCATTTAAACTGTTCTTGAAAGGAGTCCCGAAAAAGAATTGTAT
  CGTTTTTTATGGACCTGCCGATACAGGGAAGTCATACTTTTGCATGTCCCTCTTGCAATTTCTTGGGGGG
  GCTGTTATCTCATACGCTAACTCAAGTTCACACTTTTGGCTTCAGCCTTTGAGTGACTCCAAGATTGGGC
  TCCTGGACGACGCCACCCCACAGTGCTGGTCATATATTGACATCTACCTTAGGAACCTGCTCGACGGCCA
  CCCCGTTAGCATTGATAGAAAGCACAAAACTCTCCTGCAGCTCAAGTGTCCCCCCCTCATGATCACCACA
  AACACAAACCCTCTGGAAGAGGACCGCTGGAAGTACCTCCGGAGTAGACTTACAGTTTTCACATTTAAGA
  ATCCTTTCCCATTCGCTTCCCCCGGCGAGCCGCTGTACCCGATCAATAATGCCAATTGGAAGTGCTTTTT
  TCAGCGGTCTTGGTCTCGACTGGATCTCAACTCTCCCGAGGAGCAGGACGACAACGGAAACACCGGCGAG
  CCATTTCGGTGCGTTCCTGGAGATGTGGCCAGAACCGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101813 representing NP_077118
  Red=Cloning sites Green=Tags
  
  MEDSEGTDGTEEDGCRAGGWFHVEAIITHGQRQVSSDEDEDETETGEDLDFIDNRVPGDGQEIPLQLYAQ
  QTAQDDEATVQALKRKFVASPLSACSCIENDLSPRLDAISLNRKSEKAKRRLFETEPPDSGYGNTQMVVG
  TPEEVTGDEESQGGRPVEDQEEERQGGDGEADLTVHTPQSGTDAAGSVLTLLRSSNLKATLLSKFKDLFG
  VGFYELVRQFKSSKTACADWVVCAYGVYYAVAEGLKKLIQPHTQYAHIQVQTSSWGMVVFMLLRYNCAKN
  RDSVSKNMSMLLNIPEKHMLIEPPKLRSTPAALYWYKTAMGNGSEVYGETPEWIVRQTLVGHSMEDEQFR
  LSVMVQYAYDHDIVEESVLAFEYAQLADVDANAAAFLNSNCQAKYVKDAVTMCRHYKRAEREQMSMSQWI
  TFRGNKVSEEGDWKPIVRFLRHQGVEFVSFLAAFKLFLKGVPKKNCIVFYGPADTGKSYFCMSLLQFLGG
  AVISYANSSSHFWLQPLSDSKIGLLDDATPQCWSYIDIYLRNLLDGHPVSIDRKHKTLLQLKCPPLMITT
  NTNPLEEDRWKYLRSRLTVFTFKNPFPFASPGEPLYPINNANWKCFFQRSWSRLDLNSPEEQDDNGNTGE
  PFRCVPGDVARTV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001352
• ORF Size: 1929 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001352.1, NP_077118
• RefSeq ORF: 1929
• Locus ID: 1489227
• MW: 72.4 kDa