E7 (NC_001352) Virus Tagged ORF Clone

CAT#: VC101812

Myc-DDK-tagged ORF clone of viral ORF for early protein E7 [Alphapapillomavirus 4], codon optimized for human cell expression, NP_077117

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101812 represents NCBI reference of NP_077117 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGGTAACCGACCCTCCTTGAAAGATATCACCCTCATTCTCGACGAGATTCCCGAAATTGTCGACC
  TTCACTGTGACGAACAATTCGACTCCAGCGAAGAGGAAAATAATCACCAGCTGACTGAACCCGATGTGCA
  GGCCTATGGAGTGGTGACCACTTGTTGTAAATGCGGCAGAACCGTGAGACTGGTGGTGGAATGCGGCCAG
  GCCGATCTGAGAGAGTTGGAGCAATTGTTCCTCAAAACCCTGACGCTCGTCTGCCCACACTGCGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101812 representing NP_077117
  Red=Cloning sites Green=Tags
  
  MHGNRPSLKDITLILDEIPEIVDLHCDEQFDSSEEENNHQLTEPDVQAYGVVTTCCKCGRTVRLVVECGQ
  ADLRELEQLFLKTLTLVCPHCA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001352
• ORF Size: 276 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001352.1, NP_077117
• RefSeq ORF: 276
• Locus ID: 1489226
• MW: 10.4 kDa