E4 (NC_001352) Virus Tagged ORF Clone

CAT#: VC101815

Myc-DDK-tagged ORF clone of viral ORF for early protein E4 [Alphapapillomavirus 4], codon optimized for human cell expression, NP_077120

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101815 represents NCBI reference of NP_077120 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAGCCCTGGTCGCGGACGGTGCACCTGGCTTGGGCTGCTCTTCACAATACACCCCCACTTGTGCC
  TCGCCCCCAGACCGGCCCCGAGAACAACAAACCATTATCCCCTCTTGGAGCTGCTGTACCCACAGAGCCA
  GCCGCAGTCACAGCCTCAGCAGAACCAGCAGGAACAGGAAGAACAGCTCAGGCCACCAAAGCGCTGCGCA
  CCACCGAGAAGGCAGAGGGTGAGGCGACCATCCGCTAGCGTCAGCTCATCAGATTCCTCTATCCCTGGCC
  CGACCCTCCGAGAGAGGAGTGAACGCGGTAAATGGTCTGTGACTACTAGCGGAGCCAGCGTGACCCTCAC
  CGCCCAGACTCCTGGAGGCGCAACAGTCACCCTGACATTGTGCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101815 representing NP_077120
  Red=Cloning sites Green=Tags
  
  MGSPGRGRCTWLGLLFTIHPHLCLAPRPAPRTTNHYPLLELLYPQSQPQSQPQQNQQEQEEQLRPPKRCA
  PPRRQRVRRPSASVSSSDSSIPGPTLRERSERGKWSVTTSGASVTLTAQTPGGATVTLTLCL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001352
• ORF Size: 396 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001352.1, NP_077120
• RefSeq ORF: 396
• Locus ID: 1489229
• MW: 14.5 kDa