E2 (NC_001676) Virus Tagged ORF Clone

CAT#: VC101821

Myc-DDK-tagged ORF clone of viral ORF for regulatory protein [Alphapapillomavirus 13], codon optimized for human cell expression, NP_043291

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101821 represents NCBI reference of NP_043291 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACATTGGCGACTCGGCTGGACGTATGCCAGGAGCGCCTGCTCGATCTGTATGAAAAGGACTCTA
  ATAAGCTTGAAGATCAGATCGAGCACTGGAAATGTATCCGCCTGGAATGTGCCCTGCAATACAAAGCACG
  CGAAATGGGCTACAAAGTGCTCCAGCATCAGGCACTGCCAGCTCTCGCTGTTAGCAAGGGCAAGGGGCAT
  AAGGCCATTGAACTCCAGCTGGCACTCGAGACTTTGCAGAAAACCGTATACAGTACCGAGCCCTGGACTT
  TGCAGGACACCTGTCTCGAACGGTGGAACGCGCCTCCCACCGGCTGCCTGAAGCGGCGAGGCCAGACCGT
  CGATGTTATCTTCGATGGTCACCAAGATAACACCATGCAGTACGTCATGTGGGGCGACATCTACTATCAA
  AATTGCGATGGAGAAGGGTGGACCAAGGTCTGTAGCAACATTGATGCCATGGGCATATACTACATGGATG
  CCGAGCACAAGGTTTACTATGTCGATTTCAAGAAAGAGGCATCCAAATACGGCGAATATGGTCAGTGGGA
  AGTGCGGATGGGTTCCTCTATTATTTTCAGTCCAGCCAGCGTGAGTTCCACTGAAGAGGCTCTGAGTATT
  AGTAGCACAGGAACTGCAGAACATACCCGGCCAGCTAATAGCACACCCAGGACGGATAACTCAACCAAAG
  CAATACCTTGTACACCGCCCCCGCGGAAACGCGCGAGGGTATATAGCACCGACCAGCAGCCTCACAGCAC
  ATCAGATCCTGTTGGATGCGACAACGACCGGCACATTAGTGATGATAATAACAAGAATCAGGGCCGGCAT
  ACATCTTCAGGTGACACCACCCCAATCGTGCATTTTAAGGGTGAACCAAATACTCTTAAGTGCTTCAGGC
  AAAGAATCCAAAAGTACAAGCACCTGTTCGAGCAGGCTTCTTCCACCTGGCATTGGGCTTGCGTTCCGGG
  CACAACCAAAAACCGGGGGATCGTTACCCTCACCTACTCATCCGTGGAGCAGCGGCAACAGTTTCTGGTA
  ACCGTGCGGATTCCTCCGTCCATCTCCATGAGCCTCGGTGTGATGTCTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101821 representing NP_043291
  Red=Cloning sites Green=Tags
  
  METLATRLDVCQERLLDLYEKDSNKLEDQIEHWKCIRLECALQYKAREMGYKVLQHQALPALAVSKGKGH
  KAIELQLALETLQKTVYSTEPWTLQDTCLERWNAPPTGCLKRRGQTVDVIFDGHQDNTMQYVMWGDIYYQ
  NCDGEGWTKVCSNIDAMGIYYMDAEHKVYYVDFKKEASKYGEYGQWEVRMGSSIIFSPASVSSTEEALSI
  SSTGTAEHTRPANSTPRTDNSTKAIPCTPPPRKRARVYSTDQQPHSTSDPVGCDNDRHISDDNNKNQGRH
  TSSGDTTPIVHFKGEPNTLKCFRQRIQKYKHLFEQASSTWHWACVPGTTKNRGIVTLTYSSVEQRQQFLV
  TVRIPPSISMSLGVMSL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001676
• ORF Size: 1101 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001676.1, NP_043291
• RefSeq ORF: 1101
• Locus ID: 1497435
• MW: 41.7 kDa