L1 (NC_001676) Virus Tagged ORF Clone

CAT#: VC101824

Myc-DDK-tagged ORF clone of viral ORF for major capsid protein [Alphapapillomavirus 13], codon optimized for human cell expression, NP_043294

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101824 represents NCBI reference of NP_043294 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCGACCGTCAGAGAATAAGGTTTATTTGCCACCAACACCTGTTTCTAAAGTCGTTAGTACCGATG
  AGTACGTCACGCGGACAAGCATATACTACCATGCAAGCAGTTCCCGCCTGCTCGCTGTCGGCCATCCTTA
  TTTTAAAGTGCAAAAGACTAACAATAAACAGTCAATCCCTAAGGTCAGTGGATACCAGTACCGCGTGTTT
  AGGGTACAGCTTCCAGACCCGAATAAGTTTGGACTGCCTGATCCCAGTCTCTATAATCCGGAGACCCAGC
  GGCTGGTCTGGGCCTGTACCGGTGTGGAAGTGGGTCGCGGCCAGCCATTGGGTCTGGGGTTGAGTGGGCA
  CCCTCTTCTGAACAAACTGGACGACACGGAAAACGCTCCAAAATACGTCGGCGCTGGAGCAGATAATCGG
  GAAAACGTCTCTATGGATTACAAGCAAACACAGCTTTGCATCCTGGGGTGTACACCCCCTATCGGCGAGC
  ATTGGGCGAAAGGGAATCTTTGCACGCCAAACACTTTGGCCGCTGGTGACTGTCCACCACTGGAGCTCGT
  TAACAGTTACATCCAGGATGGCGACATGGTTGATATTGGCTTCGGGGCAATGGACTTCAAGACCCTGCAG
  ACCTCTAAAAGCGAGGTGCCGCTTGATGTCGCGACCTCTATATGTAAATACCCGGACTACCTGAAGATGG
  CCGCTGAGGCCTATGGAGACAGCCTGTTCTTCTACCTGAGGCGGGAACAGATGTTTGTGCGGCATATGCT
  TAACAGGGCTGGCACTATGGGCGAACCCGTACCAAATGACCTCTACATCAAAAAGTCTAGTGGGAACCTG
  GACTCTAGCATTTACGCCGCCACTCCTTCCGGATCTATGGTCACCTCTGAGTACCAAATTTTTAACAAAC
  CCTATTGGCTGCAGCGCGCTCAGGGCCAGAACAATGGCATCTGTTGGGGGAATCAGGTGTTCCTCACAGT
  AGTGGATACCACACGCTCAACTAACCTTACTCTTTGTGCAACAGCGAGTACCCAGGACTCCTTCAACAAC
  AGCGATTTTAGGGAATACATCCGGCATGTGGAGGAGTACGACTTGCAGTTCATTTTCCAGCTGTGTACTA
  TTACATTGACTGCTGATGTGATGGCATACATCCACGGCATGAATCCGACTATCCTCGAGGACTGGAACTT
  CGGTATTACGCCCCCGGCCACATCAAGCCTCGAGGACACATACCGGTTCGTTCAGTCCCAAGCAATCGCA
  TGCCAGAAGAATAATGCGCCCGCAAAGGAAAAGGAGGACCCCTACAGCAAGTTCAATTTTTGGACCGTGG
  ACCTGAAAGAACGCTTTAGCTCAGACCTCGACCAGTTTCCCCTCGGCCGGAAGTTCCTGCTGCAGGCTGG
  CCTCAGGGCTCGGCCCCGGCTGAGACCTGTGAAAAGGGCTGCCCCCAGCAGCTCAAAGGGAACCGCACGA
  AAGAGGGCAAAAACCAAGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101824 representing NP_043294
  Red=Cloning sites Green=Tags
  
  MWRPSENKVYLPPTPVSKVVSTDEYVTRTSIYYHASSSRLLAVGHPYFKVQKTNNKQSIPKVSGYQYRVF
  RVQLPDPNKFGLPDPSLYNPETQRLVWACTGVEVGRGQPLGLGLSGHPLLNKLDDTENAPKYVGAGADNR
  ENVSMDYKQTQLCILGCTPPIGEHWAKGNLCTPNTLAAGDCPPLELVNSYIQDGDMVDIGFGAMDFKTLQ
  TSKSEVPLDVATSICKYPDYLKMAAEAYGDSLFFYLRREQMFVRHMLNRAGTMGEPVPNDLYIKKSSGNL
  DSSIYAATPSGSMVTSEYQIFNKPYWLQRAQGQNNGICWGNQVFLTVVDTTRSTNLTLCATASTQDSFNN
  SDFREYIRHVEEYDLQFIFQLCTITLTADVMAYIHGMNPTILEDWNFGITPPATSSLEDTYRFVQSQAIA
  CQKNNAPAKEKEDPYSKFNFWTVDLKERFSSDLDQFPLGRKFLLQAGLRARPRLRPVKRAAPSSSKGTAR
  KRAKTKR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001676
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001676.1, NP_043294
• RefSeq ORF: 1491
• Locus ID: 1497438
• MW: 55.8 kDa