E1 (NC_014953) Virus Tagged ORF Clone

CAT#: VC101864

Myc-DDK-tagged ORF clone of viral ORF for E1 [Human papillomavirus type 129], codon optimized for human cell expression, YP_004169272

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101864 represents NCBI reference of YP_004169272 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACAACCCTAAGGGTATCAAAGATAAGACGGAGTGCTTTGTTGAGTTCGAAGCAGAGTGTGTAG
  ACGCTACTAACACACTTGATGAGCTGTTTGACGGCTCTACAGATGACGGATCTGATATCTCCAACTTGAT
  CGATGACTCAGTGGTGGAGCAAGGTAACTCCCTGGCCCTTTTCAATGTGCACGTGACCGAAGAATGCGAC
  AAGGCAGCAAGCGTTTTGAAACGGAAATATGCCAAGAGCCCCAAAGGTCCATCAGTAGCTGACATATCTC
  CACGCCTCGAAGCCATCTGTATCAGCCCTGAGAAAGAGAGACTGTCAAAAAGGAGACTGCTCTTCGAGGA
  CTCAGGCATCGTGGAGGATGAAGCTGAGAACCATAATGTCCAGGTAGATACACAGGAAGACGAGAATGGT
  GGCGACACTCGCGTGGCAGAGGCCATCCCTAATATTATAAAGAACTTGTTTAAATCAAACTGCAAGAGGA
  GTTACCTTCTGAACAAATTCGAAAACATGTTCGGCATCCCTTACACGGAGCTTGTACGCCACTTTAAGAG
  TAATAAGACATGTTGTGACAACTGGGTGGTGTACGCCTTCGCCGTGAACGAAGAAGTTTTGCAGTCCAGT
  AAAATTCTCCTGCAACAGCACTGCGAGTGTATGCAAATCGTGTTGCAACCATTCTGTGGCCTGTTTCTGC
  TTCAGTTTAAACATGCTAAGAGTCGGGAGACTGTCGGCAACCTTTTCTGCAATACCCTGAATGTCAAGGA
  ATGCCAGCTGCTGTGTGACCCTCCAAAGAGTCGGTCTGTGCCTGCTGCTCTGTATTTCTACAAGAAAGAA
  CATACAGAGACATCATTTGTGTACAACAAGCTCCCATCTTGGGTGACCCAATTGACCCTCATCAGTCACC
  AGACTGCTGCCCAGCCAGAGAACTTTGAGCTGTCTAAAATGATCCAATGGGCGTATGACAACAAGAAGGT
  CGAAGAGAGCGAAATTGCCTACGGGTACGCCAGCATCGCCGATGAGGACTCCAACGCCGCCGCCTTTCTG
  AAATCAAACTGCCAGATGAAATACGTCAGAGACTGCAGCAATATGGTGCGGTTGTACCTGCGCCAGGAGA
  TGCGGGACATGTCTGTCAGTCAGTGGATTTGGAAGTGTTGTAACGACTGTGAAACAGAAGGGGACTGGAA
  GGTCGTGAATAACTTCCTTAAGTATCAGCAAGTAAATATTCTGAGCTTTCTTAGCACACTTCGATTGTTC
  TTTAAGAGCATCCCTAAAAAGAATTGTCTCGTCATATACGGCCCTCCTGACACAGGCAAAAGTTACTTTT
  GCTACTCCCTCATATCATTTTTTAAAGGTAAAATTATCAGTTATATGAACCGCTCTTCAACATTTTGGTT
  GCAGCCACTTATTGATTGTAAGTTCGGACTGTTGGACGACGCTACACATGCATGCTGGCGGTACATCGAC
  GAAAACATGCGGAACGTGCTGGATGGGAACGTAATGTGTGCGGACGCCAAGCATAGAGCGCCCCAACAGC
  TTCACTTGCCTCCGCTGATCGTGACTACGAACGTTGATGTCCTGGAGGACATATCATTGAAATACCTGCA
  TTCACGAATAACTTGCATTAAGTTCGCTCAGAAGATGCTGTTCGACGACAATGGCGATCCTTTTTACAAG
  TTCACTAATGCAGTCTGGAAGAGCTTCTTTAAGCGCTTGTGGAAGCAGCTGGATCTCGAAGAAGAAGAAA
  ACGAGCCCACAGGGTCCGACAGAATGTTCAGATGCACTGCCGAATGTCCTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101864 representing YP_004169272
  Red=Cloning sites Green=Tags
  
  MADNPKGIKDKTECFVEFEAECVDATNTLDELFDGSTDDGSDISNLIDDSVVEQGNSLALFNVHVTEECD
  KAASVLKRKYAKSPKGPSVADISPRLEAICISPEKERLSKRRLLFEDSGIVEDEAENHNVQVDTQEDENG
  GDTRVAEAIPNIIKNLFKSNCKRSYLLNKFENMFGIPYTELVRHFKSNKTCCDNWVVYAFAVNEEVLQSS
  KILLQQHCECMQIVLQPFCGLFLLQFKHAKSRETVGNLFCNTLNVKECQLLCDPPKSRSVPAALYFYKKE
  HTETSFVYNKLPSWVTQLTLISHQTAAQPENFELSKMIQWAYDNKKVEESEIAYGYASIADEDSNAAAFL
  KSNCQMKYVRDCSNMVRLYLRQEMRDMSVSQWIWKCCNDCETEGDWKVVNNFLKYQQVNILSFLSTLRLF
  FKSIPKKNCLVIYGPPDTGKSYFCYSLISFFKGKIISYMNRSSTFWLQPLIDCKFGLLDDATHACWRYID
  ENMRNVLDGNVMCADAKHRAPQQLHLPPLIVTTNVDVLEDISLKYLHSRITCIKFAQKMLFDDNGDPFYK
  FTNAVWKSFFKRLWKQLDLEEEENEPTGSDRMFRCTAECPE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014953
• ORF Size: 1803 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014953.1, YP_004169272
• RefSeq ORF: 1803
• Locus ID: 10146733
• MW: 69.1 kDa