E7 (NC_014953) Virus Tagged ORF Clone

CAT#: VC101868

Myc-DDK-tagged ORF clone of viral ORF for E7 [Human papillomavirus type 129], codon optimized for human cell expression, YP_004169271

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101868 represents NCBI reference of YP_004169271 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGCAAACATAGCACGATCAAGGACATCAACCTCGAATTGAGTGAGCTCGTAATACCAGAAAACC
  TCCTGTGCAACGAATCTTTGTCTACAGATGAGGAGCCCGAAGAGGAGCAAGTAACTCCTTACCAGGTCGA
  CAGCTGGTGTCCAAATTGCAATAAAGGAGTGCGCGTTTGTGTTCTGGCGACCTCATTCGCAATTCAGGCC
  TTTCAGAGACTGTTGACACAGCAGCTGCAGTTCGTCTGCCCAGGATGCAGTAGAGTGATTTTTAGGAACG
  GACGACAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101868 representing YP_004169271
  Red=Cloning sites Green=Tags
  
  MQGKHSTIKDINLELSELVIPENLLCNESLSTDEEPEEEQVTPYQVDSWCPNCNKGVRVCVLATSFAIQA
  FQRLLTQQLQFVCPGCSRVIFRNGRQS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014953
• ORF Size: 291 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014953.1, YP_004169271
• RefSeq ORF: 291
• Locus ID: 10146728
• MW: 11.0 kDa