E6 (NC_014953) Virus Tagged ORF Clone

CAT#: VC101867

Myc-DDK-tagged ORF clone of viral ORF for E6 [Human papillomavirus type 129], codon optimized for human cell expression, YP_004169270

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101867 represents NCBI reference of YP_004169270 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAAGATCCAGCAGTTCCTCCCTCACCTTATGGAAAATCTGTTCCCTACCGGCCTGCGAGATTACT
  GTTCCTACTTCGACATACCTTTCTTCGATCTGAAACTTCCATGCATTTTCTGTAAGAATATCTGCAACCT
  GGTGGATCTCGCTCAGTTCTTCGAAAAGAAACTGTGCATCGTGTGGAGACAGCATGTGGCCCACGCCTGC
  TGTGAGAAATGCCTGACACTCTGCGCGCGATATGGTTCCGAGCATCACTGCGTGTGTAGCGTAAAGGCAG
  AGAATCTGCACGGCCTGATTAGGCAGCCACTTCAAAACATACTCCTGCGCTGTAATTATTGCTTCCGCGT
  ATTGACTGCCTCAGAAAAAGTAGACCTGGTCACAGGTGGGCGGTATTGCTGGCTCGTGCGGGGTCACTGG
  AGAGCCATCTGTTCTCTGTGTGCAACCAAGGAATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101867 representing YP_004169270
  Red=Cloning sites Green=Tags
  
  MWKIQQFLPHLMENLFPTGLRDYCSYFDIPFFDLKLPCIFCKNICNLVDLAQFFEKKLCIVWRQHVAHAC
  CEKCLTLCARYGSEHHCVCSVKAENLHGLIRQPLQNILLRCNYCFRVLTASEKVDLVTGGRYCWLVRGHW
  RAICSLCATKEL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014953
• ORF Size: 456 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014953.1, YP_004169270
• RefSeq ORF: 456
• Locus ID: 10146734
• MW: 17.8 kDa