E2 (NC_014954) Virus Tagged ORF Clone

CAT#: VC101872

Myc-DDK-tagged ORF clone of viral ORF for E2 [Human papillomavirus type 131], codon optimized for human cell expression, YP_004169280

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101872 represents NCBI reference of YP_004169280 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGATGGAGACCCAGGAGTCTCTCAGCGTACGGTTCGCTGCACAGCAAGAGGTGCAGCTTAGTCTGA
  TAGAGAAAGAGTCTACAGATTTGAGAGATCACATTACTTACTGGGATGCCGTCCGAAAAGAAAATGTCAT
  CGTGTTCTACGCCAAGAAGGAAGGGTACTCAAATCTGGGACTCCAGCCTCTTGCCACAACAGCTGTAAGT
  GAACACCGCGCCAAACAGGCAATTAAAATCCAGTTGCTCCTCGAGGGCCTCATCAAAAGCCCTTACGGCA
  ATGAAACATGGACCCTGGGCGAGGTCTCAGCTGAGCTGCTGAATAGCGAGCCGAAAAATTGCTTCAAGAA
  GGGCGGATTCATCGTGACAGTGCTGATCGATAACGATCCCAAGAATATCTACCCTTACACATGTTGGGAA
  TATATTTACTACCAAGATGTTAACAATGATTGGCACAAGGTAATGGGACAGGTTGACCACAACGGCCTGT
  ACTTTAAGGAGCACACAGGCGACATTGTCTACTTCGTCGTATTCCAACCCGATGTGCTCAAGTATGGAAA
  AACAGGCGAATGGACCGTAAAATTCAAGAACAAAACCATCCTGGCTCCAGTGACTTCCAGTTCAAGGACA
  TTCTTCGAACCCTCCACCAGTCAGACAACTGAACAGCCCTCAACATCAAACACTACTTACGGCCCTGAGA
  GTCCCCGGAGAAGGCAACAAACCAGGGAGGATACTGAATCCCCTACAAGCACCAGCTCCGGATTGCGGCT
  GCGGCGGCGGAAGAGGGGAGAGTCACCAACCTCATCAACGAGGGTCAAGAAAAGCAGGCTGGGCGCCGAT
  TCAGCTCCTACTCCCAGCGAGGTTGGATCACGGTCTCATTCCGTGGCCAGACATGGACTGTCTCGCCTCG
  GCCGGCTCCAAGAAGAAGCTAGGGATCCTCCAGTCGCTTTGCTTACGGGACACCCCAACAATCTTAAGTG
  CTGGCGGAATCGGTGCAACCAGAAGTATGGGCACTTGTACCTCTGTTGCTCCTCAGTGTTTAGATGGCTC
  GGCCATGACTGCGAGAAGACCGAGACCTCCAAGGTACTCGTGGCCTTCAAGGATCTGACCCAGCGACAAC
  TGTTTCTGGAAACAGTCACTCTTCCTAAAGGTACAGAAGTGACACTGGGAAGTCTCGATAGCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101872 representing YP_004169280
  Red=Cloning sites Green=Tags
  
  MQMETQESLSVRFAAQQEVQLSLIEKESTDLRDHITYWDAVRKENVIVFYAKKEGYSNLGLQPLATTAVS
  EHRAKQAIKIQLLLEGLIKSPYGNETWTLGEVSAELLNSEPKNCFKKGGFIVTVLIDNDPKNIYPYTCWE
  YIYYQDVNNDWHKVMGQVDHNGLYFKEHTGDIVYFVVFQPDVLKYGKTGEWTVKFKNKTILAPVTSSSRT
  FFEPSTSQTTEQPSTSNTTYGPESPRRRQQTREDTESPTSTSSGLRLRRRKRGESPTSSTRVKKSRLGAD
  SAPTPSEVGSRSHSVARHGLSRLGRLQEEARDPPVALLTGHPNNLKCWRNRCNQKYGHLYLCCSSVFRWL
  GHDCEKTETSKVLVAFKDLTQRQLFLETVTLPKGTEVTLGSLDSL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014954
• ORF Size: 1185 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014954.1, YP_004169280
• RefSeq ORF: 1185
• Locus ID: 10146738
• MW: 44.8 kDa