E7 (NC_014954) Virus Tagged ORF Clone

CAT#: VC101875

Myc-DDK-tagged ORF clone of viral ORF for E7 [Human papillomavirus type 131], codon optimized for human cell expression, YP_004169278

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101875 represents NCBI reference of YP_004169278 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGGAGAAAAGGCCACCATTCCAGACATCGAGCTGGCCGAGCTCGTCCTTCCTTGTTCTCTGCTGT
  CTGATGAGAGCCTTTCAGCCGACGACACGCCAGAGGAAGTGGAACTCTCACCCTACCGAATAGATTCAAA
  CTGTAGTAACTGCCACACCAACATCCGGCTGTGTGTGTCCGCCTCCACTAGCGCTATTCGGATCCTGCAT
  CAGCTGCTTCTCAGCGATCTCCACCTGCTTTGCAGCGGTTGCTCCAGGTTCAGTCTGAGGGATGGGCGAT
  CA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101875 representing YP_004169278
  Red=Cloning sites Green=Tags
  
  MRGEKATIPDIELAELVLPCSLLSDESLSADDTPEEVELSPYRIDSNCSNCHTNIRLCVSASTSAIRILH
  QLLLSDLHLLCSGCSRFSLRDGRS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014954
• ORF Size: 282 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014954.1, YP_004169278
• RefSeq ORF: 282
• Locus ID: 10146736
• MW: 10.3 kDa