E4 (NC_014955) Virus Tagged ORF Clone

CAT#: VC101880

Myc-DDK-tagged ORF clone of viral ORF for E4, partial [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169288

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101880 represents NCBI reference of YP_004169288 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  AGTATTAAGGTTAAATTGTTCCTGCCTCTGTGTCTGGCCAGCCAGGGTCCTTTTCAGAGTCTGAGACAAC
  GGGTGCCTTCACCCCCCCGGACGCCACATCCGCTTAGGAAGAGCCTGGAGAGAGACGGCGAAAGACCAAG
  ACGCACCCCGACGCCATCACGACCTCCACGACCCACTCTGAATTTCGATGACGATGAGGAAACCAATAAG
  GAGAACCAACCACCCGATTATGAGGAAGAGCAACTCGGCGCTGCCGCTACCCTGCATCAGCTTCTCAAGA
  AGTGGGGTCAGGAGCTGGACCAGTTCCAGAACACAGTGTGCAGAGATCTGGATGACTTTAAGAAAAGACT
  GGGTATACGCCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101880 representing YP_004169288
  Red=Cloning sites Green=Tags
  
  SIKVKLFLPLCLASQGPFQSLRQRVPSPPRTPHPLRKSLERDGERPRRTPTPSRPPRPTLNFDDDEETNK
  ENQPPDYEEEQLGAAATLHQLLKKWGQELDQFQNTVCRDLDDFKKRLGIRH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014955
• ORF Size: 363 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014955.1, YP_004169288
• RefSeq ORF: 363
• Locus ID: 10146747
• MW: 14.1 kDa