E2 (NC_014955) Virus Tagged ORF Clone

CAT#: VC101879

Myc-DDK-tagged ORF clone of viral ORF for E2 [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169287

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101879 represents NCBI reference of YP_004169287 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGATGGAGACACAAGAGACTCTGACGGCCCGCTTTGTCGCTCAGCAGGAAATCCAGCTGGCCCTGA
  TCGAAAAGGAAAGCACAAACCTGCAGGATCATATCCAGTACTGGTCAGCTGTTCGGAAAGAACACGTCCT
  TGCCTACTACGCGCGACAGGAGGGGTATACTCGGCTTGGGCTGCAGCCTCTGGCAAGCACAGCCGTCTCC
  GAATACCGGGCCAAAGAAGCCATTCACATTGTGATTCTCCTCAAATCCCTGCTGAAATCCGAGTATGGCC
  CAGAACTCTGGACTCTCGCTGAAGTAAGTGCCGAAATTTTGAATACTCCTCCTAAGAACTGTTTCAAAAA
  AGGTCCATTCACCGTCACCGTCTACTTCGACAATGACACAAACAACAGCTTCCCCTATACATGTTGGAAC
  TACATTTATTATCAGGATGATAACAACCGGTGGCACAAGACCGAAGGGCAAGTTGACGCCAACGGTCTCT
  ATTTTAAGGAAAAGAACGGCGACGTCACTTATTTTACCGTCTTCCAGCCAGACGCCGAAAGATACGGACA
  TACTGGCCAGTGGACCATCAAGTATAAGTCTCAGACCATCTCCGCATCTGTTTCTTCATTTAGCCGGTCT
  GTCCCTGAACCTCCTCCGGAGAGCCCGGAACCATCCCCCGATACCCCATCAACATCAAAAGTTTCCGGTA
  AGCGCAGAAGAGAGACAGAGGAAGATTCACACCCGGAGAGCCCAAGTTCAAGCAATATAGAACTCAGGAG
  AAGAAGGGGCAACCAGCAGAGAAAAAGCACCACCAGACTGAGAGGAGGGGCAGCGGGTCGCTCCAGCAAC
  AGCGCACCAACACCTGAAGAGGTTGGTGCAGGAACTAGATCCGTCCCCAAGCACGGACTTAGCGGACTGC
  GGAGGCTCCAGGAGGAAGCTCGCGACCCGCCTCTCATCCTTCTGAAAGGGCATCCTAATACCTTGAAATG
  TTGGAGGTGGCGGTGTAAAAACAAGTACTCACACCTGTTTCTCGCGTGCACTACCGTTTTCCACTGGGTC
  GGCGATGACACAAATAAATCTACCAGCGCCCGGGTGCTGCTGGCGTTCGCCACGGTCTGCGAGCGCCAGA
  CATTTCTTAATACAGTCCCCCTGCCCAAAGGAACCTCCTATGTACTGGGGAATCTGGATTCACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101879 representing YP_004169287
  Red=Cloning sites Green=Tags
  
  MQMETQETLTARFVAQQEIQLALIEKESTNLQDHIQYWSAVRKEHVLAYYARQEGYTRLGLQPLASTAVS
  EYRAKEAIHIVILLKSLLKSEYGPELWTLAEVSAEILNTPPKNCFKKGPFTVTVYFDNDTNNSFPYTCWN
  YIYYQDDNNRWHKTEGQVDANGLYFKEKNGDVTYFTVFQPDAERYGHTGQWTIKYKSQTISASVSSFSRS
  VPEPPPESPEPSPDTPSTSKVSGKRRRETEEDSHPESPSSSNIELRRRRGNQQRKSTTRLRGGAAGRSSN
  SAPTPEEVGAGTRSVPKHGLSGLRRLQEEARDPPLILLKGHPNTLKCWRWRCKNKYSHLFLACTTVFHWV
  GDDTNKSTSARVLLAFATVCERQTFLNTVPLPKGTSYVLGNLDSL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014955
• ORF Size: 1185 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014955.1, YP_004169287
• RefSeq ORF: 1185
• Locus ID: 10146746
• MW: 44.8 kDa