E6 (NC_014955) Virus Tagged ORF Clone

CAT#: VC101881

Myc-DDK-tagged ORF clone of viral ORF for E6 [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169284

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101881 represents NCBI reference of YP_004169284 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCTACAGACCTAGTAACGTTGCAGAGTATTGTGCATTCTATAATCTCAACTTTACCGACGTGG
  GGATGCGATGTTGCTTCTGTAAGGCCTGGCTGAACTGCGTGGAGCTGGCCTCCTTTCATTATAAAAGTCT
  CTCACTGCTCTGGAGAGATAATAATGTGTTCGCTTGTTGTTGCCAGTGCTTGCGGCTGAGTGCAAAGTAT
  GAGATGGAGCGCTTTTATCGCTGTTCCGTGAAGGCCAAAATCATCGAGGATGTCGTGCAGACACCCCTGA
  TCGAGATCGTGATACGGTGTTTTCATTGCTTTGCCCTCCTGGACCTGATTGAGAAAATCGAACATAAGTA
  CAGAGACGATTCATTCCACCTTGTTCGGCACTTCTGGAGAGGTGAATGTAGACATTGTTGCCGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101881 representing YP_004169284
  Red=Cloning sites Green=Tags
  
  METYRPSNVAEYCAFYNLNFTDVGMRCCFCKAWLNCVELASFHYKSLSLLWRDNNVFACCCQCLRLSAKY
  EMERFYRCSVKAKIIEDVVQTPLIEIVIRCFHCFALLDLIEKIEHKYRDDSFHLVRHFWRGECRHCCRK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014955
• ORF Size: 417 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014955.1, YP_004169284
• RefSeq ORF: 417
• Locus ID: 10146750
• MW: 16.7 kDa