E1 (NC_014835) Virus Tagged ORF Clone

CAT#: VC101892

Myc-DDK-tagged ORF clone of viral ORF for E1 [Human papillomavirus type 148], codon optimized for human cell expression, YP_004111311

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101892 represents NCBI reference of YP_004111311 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGAACCGCATAAAGGGACCTCCAATATAGACTGTCTTGAAGATATCAACGAGTGGTGCTTCATCA
  CAGAAGCCGATTGTGTGGATAGTGCCGAGACTCTCAGTGAGCTCTTCGATGCTGACACCGATGCCTCTAA
  TATTTCCAATTTGATCGATGATGATGACTTGGACTCCCTGAGTCAGGGAAACAGCCTCGCCCTCTTCAAC
  AACCAGGTCGCGGAAGAATGTGACCGGGCAATTACTAATCTGAAGAGAAAGTTCATCGCATCCCCCGAGC
  ACATTGTCGAGAAGTCCTCACCCCGATTGCAAGCGGTGCACATATCCCCTCAGCGAAAAAGCATTTCTAA
  AAGGAAACTTTTTGAGGACAGTGGGATAGAGCACGACGAAACCACAGATTCTGTAGTGCAGGTGGAGCCC
  TTGGAGAACAGTAACAACGGGGCAACTCAGGAGGAAAATGCCATCCTGCAATCTTCCAACGTGAAGGCAA
  CCTTGTACAGTAAGTTCAAGGAGCGGTTCAACGTGAGTTTCACGGAAATCTCAAGAAACTTCAAGAGCGA
  CAAGACATGTAATCATAACTGGTTGATCGTGGCATTTGCTGTTCAGGATGATTTGATTGAGGCCTCTAAA
  ACTCTGATGCAGCAACATTGCGACTACCTGCAAATGATCATTTCTGGCTTCACCGTTCTTTACCTCGTTG
  AGTTCAAATCCACGAAAAACAGAGAGACTGTCCTTAAGCTTATCACCCAGATGCTGAATGTGAATGAACG
  GCAAGTGCTGTGCGAGCCTCCTAGAATACGCTCCACAGCAACAGCTATGTTTTTTTACAGGAAGGCTCTG
  GCCAATATTGGTTTCAAATGCGGAGCCTACCCCCAGTGGATGGTCAAACTGACCATGGTCGATCACCAAT
  TCGCCGCTGCTGAGACATTCAAACTGTCAGAAATGGTACAGTGGGCCTATGATAACGACTATACGGAAGA
  GTCTCAGATTGCCTTTTATTATGCTCAGTACGCCGATATCAACAGCAACGCAGCCGCCTTCCTGGAGTCC
  AACCAGCAGGTGAAATATGTCAGGGACTGTTGCAGCATGGTTCGGCTGTACAAGCGGCAAGAACTCAGGA
  ATATGTCCATGGGCGCCTGGATTAGGAAATGCTGCTCCACAGTCCCAGAGGGAGATGGGTGGAAAGAAAT
  CGCCAAATTTCTGAAATTTCAACATGTGAATATGCTTAGTCTGCTCATCGCCTTGAAACAGTTCTTCAAA
  AACATCCCGAAGAAGTCTTGCATTGTTATCTACGGCCCTCCTGATAGCGGAAAGAGCTATTTCTGTTTCT
  CTCTGATCAGGTTGCTCCAGGGTAAAGTGGTGAGCTTCATGAATAGAAATAGTCACTTTTGGACACAGCC
  TTTGATCGATGCTAAGGTAGGGATGCTCGATGATGCCACATTCCCCTGTTGGACCTTCCTTGATACATAC
  ATGCGGAATGCCTTCGATGGAAATTCTGTGAGCATTGACGTGAAACACAAGAATTTGCAGCAGATTAAAT
  TGCCACCCATGATTATTACTACCAATATTGCGGTGCCCAAGGAGCCCACTCTGAAGTACCTCCAGTCTCG
  GCTCACCTGCTTCGAATTTCCTAACACCATGCCCTTCAACGATGTGGGCAAGCCTCTCTTTAATATCACA
  ACTGAGTGCTGGGCGATGTTCTTCAGTAAGTTCTTTAGACAGCTTGAGTTGGAAGAGGACGATGGGGACT
  CTGCAGACACCGATCGGACGTTCTGCTGCACTGCAAGGAATGCAAACGATTCAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101892 representing YP_004111311
  Red=Cloning sites Green=Tags
  
  MGEPHKGTSNIDCLEDINEWCFITEADCVDSAETLSELFDADTDASNISNLIDDDDLDSLSQGNSLALFN
  NQVAEECDRAITNLKRKFIASPEHIVEKSSPRLQAVHISPQRKSISKRKLFEDSGIEHDETTDSVVQVEP
  LENSNNGATQEENAILQSSNVKATLYSKFKERFNVSFTEISRNFKSDKTCNHNWLIVAFAVQDDLIEASK
  TLMQQHCDYLQMIISGFTVLYLVEFKSTKNRETVLKLITQMLNVNERQVLCEPPRIRSTATAMFFYRKAL
  ANIGFKCGAYPQWMVKLTMVDHQFAAAETFKLSEMVQWAYDNDYTEESQIAFYYAQYADINSNAAAFLES
  NQQVKYVRDCCSMVRLYKRQELRNMSMGAWIRKCCSTVPEGDGWKEIAKFLKFQHVNMLSLLIALKQFFK
  NIPKKSCIVIYGPPDSGKSYFCFSLIRLLQGKVVSFMNRNSHFWTQPLIDAKVGMLDDATFPCWTFLDTY
  MRNAFDGNSVSIDVKHKNLQQIKLPPMIITTNIAVPKEPTLKYLQSRLTCFEFPNTMPFNDVGKPLFNIT
  TECWAMFFSKFFRQLELEEDDGDSADTDRTFCCTARNANDSN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014835
• ORF Size: 1806 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014835.1, YP_004111311
• RefSeq ORF: 1806
• Locus ID: 10067313
• MW: 68.9 kDa