E6 (NC_014835) Virus Tagged ORF Clone

CAT#: VC101895

Myc-DDK-tagged ORF clone of viral ORF for E6 [Human papillomavirus type 148], codon optimized for human cell expression, YP_004111309

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101895 represents NCBI reference of YP_004111309 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACACCAACGGGAAGCCTTGACGTATACTGCAAAGAACATGGTATCAGTTTTTTCGAATTGAGAA
  TCTCATGCATATTTTGCAGGTTTGTGTGTTCTTTGGTAGATCTCGCGGCATTCTACAATAAGCGCCTGTC
  TCTGGTTTGGAAAGACAAGGTATGCTACGCATGCTGCTCCGCATGTCTCACACTCAGCGCGAAATATGAA
  TTGGAAAACTACTACCAGTGCAGCCTCTCAAGCGACTATTTTGAAGACATAGTGAGACAACCCCTGCAGG
  AAGTGGTGATCCGGTGTCTCAAATGCCTGACCGCCCTTGATTACATGGAAAAGCTTGACCATAAGAGGAA
  TTGCAAACCCTTTCACCTTATCCGCGGACGATGGAGGGGCGATTGCAGGAACTGTGCTCAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101895 representing YP_004111309
  Red=Cloning sites Green=Tags
  
  METPTGSLDVYCKEHGISFFELRISCIFCRFVCSLVDLAAFYNKRLSLVWKDKVCYACCSACLTLSAKYE
  LENYYQCSLSSDYFEDIVRQPLQEVVIRCLKCLTALDYMEKLDHKRNCKPFHLIRGRWRGDCRNCAQK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014835
• ORF Size: 414 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014835.1, YP_004111309
• RefSeq ORF: 414
• Locus ID: 10067318
• MW: 16.2 kDa