E2 (NC_014835) Virus Tagged ORF Clone

CAT#: VC101893

Myc-DDK-tagged ORF clone of viral ORF for E2 [Human papillomavirus type 148], codon optimized for human cell expression, YP_004111312

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101893 represents NCBI reference of YP_004111312 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACACAACAGACTTTGACAGAGCGGTTTGCCGCTCAGCAAGAAATGCAGATGACGCTCATTGAAC
  AGGAGTCTACGGACCTGCGAGACCACATCAAATATTGGGACTCCGTCAGACTCGAAAATCTTTTGGCCTA
  CTACTCACGAAAAGAGGGATATACCCACCTGGGTCTCCAACCACTCCCTGCTCTGACCGTGTCCGAGTAT
  AAGTGTAAAGAAGCAATCAAAATGAAGTTGCTGTTGGAATCTCTGAAGGATTCTAGGTACGGGTATGAAC
  CATGGACACTCTCAGAGGTGAGTGCTGAAATCATCAACACCAACCCCCGAAATGCCTTCAAGAAGAAGGC
  CTTTACGGTGACGGTGTTCTTCGACAACGACCAGAGCAACAGTTTTCCATACATATGCTGGGACCACATT
  TACTACCAGGACGAGAAGTCAGAGTGGCAGAAGGTAGCCGGACAGGTGGATATCAACGGCTTGTATTTTC
  GCGAGGTCACCGGCGATATTACCTATTTCACCTTGTTTCAGCCTGACGCCGAGAGGTATGGGCAGACTGG
  CCAATGGAGCGTGACCTACAAAAACCATACACTTAGTACGAGTGTTACAAGCAGTAGCCGGACCGCTTCA
  GACCTGGAGAATAGACCTCCAACTCACCCAATAAGCTCACCCAAGACGCCGCGGAAGAGACGGAGACAGA
  CTGGGGAGGACACAACGCGCGAGAGTCCCACGAGCACTTCAAGTGGCTTGCGCTTTCGGCGCCGCCGCAC
  CGAGCAGGGGGAGCCTACAACTGATGGGTATAACACTAGGAGCACCCCGAGAAGGCGGAGAGCCGCTGTG
  GGCAGCGCGGTGAGCCCCGAAGAGGTGGGCTCTGGAACCCGCTCAGTGCCCAGAACAGGTCTGACAAGGA
  ACCAGCGACTGCAAGCCGAGGCTAGGGATCCCACGCTCATTCTGTTGCAGGGCTGTGCCAATTCCCTTAA
  ATGTTTTCGCTATCGCTGCTCCCACAAACATTCCCACCTCTATTTGGCGGCCTCTACTGTCTTTGCCTGG
  ATGTATAACAGCAATGAGAAAAGCGCCGAGGGTAGGATGCTGATTGCATTCTCAAGCAATACCCAGAGAG
  ACGTATTCTTGAAGAGTGTGACTATCCCCAAGGGTTGCCATTACTGTTTCGGAAATCTGGATTGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101893 representing YP_004111312
  Red=Cloning sites Green=Tags
  
  METQQTLTERFAAQQEMQMTLIEQESTDLRDHIKYWDSVRLENLLAYYSRKEGYTHLGLQPLPALTVSEY
  KCKEAIKMKLLLESLKDSRYGYEPWTLSEVSAEIINTNPRNAFKKKAFTVTVFFDNDQSNSFPYICWDHI
  YYQDEKSEWQKVAGQVDINGLYFREVTGDITYFTLFQPDAERYGQTGQWSVTYKNHTLSTSVTSSSRTAS
  DLENRPPTHPISSPKTPRKRRRQTGEDTTRESPTSTSSGLRFRRRRTEQGEPTTDGYNTRSTPRRRRAAV
  GSAVSPEEVGSGTRSVPRTGLTRNQRLQAEARDPTLILLQGCANSLKCFRYRCSHKHSHLYLAASTVFAW
  MYNSNEKSAEGRMLIAFSSNTQRDVFLKSVTIPKGCHYCFGNLDCL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014835
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014835.1, YP_004111312
• RefSeq ORF: 1188
• Locus ID: 10067314
• MW: 45.5 kDa